Recombinant human interleukin 17A enhances the anti-Candida effect of human oral mucosal epithelial cells by inhibiting Candida albicans growth and inducing antimicrobial peptides secretion
Recombinant human interleukin 17A enhances the anti-Candida effect of human oral mucosal epithelial cells by inhibiting Candida albicans growth and inducing antimicrobial peptides secretion
复制标题
Rh IL 17A 通过抑制白色念珠菌生长和诱导抗菌肽分泌来增强人口腔粘膜上皮细胞的抗念珠菌作用
DOI：
10.1111/jop.12889
复制
发表时间：
2020-04-01
影响因子：
3.3
通讯作者：
Wu, Tiantian
中科院分区：
文献类型：
--
作者：
Jiang, Lanlan;Fang, Meifei;Wu, Tiantian
背景白色念珠菌是引起机会性感染的最常见的真菌病原菌。白介素17(IL17A)是抗真菌免疫的重要介质。为探讨重组人白介素17A(RhIL17A)对口腔黏膜上皮细胞抗白念珠菌感染的作用。方法将口腔黏膜上皮细胞分为4组：C白念珠菌阳性组(MOI=0.1)、rhIL17A+组(100mUg/L)、rh IL17A+C白念珠菌+组(MOI=0.1)和空白对照组。24小时后观察白念珠菌生长情况。用酶联免疫吸附试验和逆转录聚合酶链式反应分别检测培养上清液中的人β-2防御素(HBD-2)、S100A8和LL-37及其在细胞中的mRNAs。而在rhIL17A+C白念珠菌+组中，IL17既抑制了菌丝的形成，又抑制了白念珠菌感染hOMEC及其进一步生长。白色念珠菌对hOMECs的粘附率无统计学意义。与对照组相比，hBD-2基因表达水平升高，其中rh IL17A+C白念珠菌+组hBD-2和hBD-2基因表达水平最高。RhIL17A+组HBD-2和HBD-2mRNA水平均高于C白念珠菌+组。结论重组人白介素17A可能通过影响白念珠菌的生长繁殖和菌丝的形成来抑制白念珠菌感染hOMECs。此外，rhIL17A还可诱导hOMEC分泌HBD-2、S100A8和IL-37，增强其抗感染能力。
Background Candida albicans (C albicans) is the most common fungal pathogen causing opportunistic infections. IL17 (IL17A) is a vital mediator of antifungal immunity. The aim of the study was to investigate the effect of recombinant human interleukin 17A (rhIL17A) on human oral mucosal epithelial cells (hOMECs) defending against C albicans infection.Methods Human oral mucosal epithelial cells were divided into four groups: C albicans+ (MOI = 0.1), rhIL17A+ (100 mu g/L), rhIL17A + C albicans+ (MOI = 0.1, rhIL17A:100 mu g/L) and blank control. Then, C albicans growth was observed after 24 hours. Human beta-2 defensin (hBD-2), S100A8 and LL-37 in supernatants and their mRNAs in cells were measured by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction, respectively.Results In C albicans+ group, C albicans hyphae formation and the death of infected hOMECs were observed. However, in the rhIL17A + C albicans+ group, IL17 inhibited both hypha formation, and C albicans from infecting hOMECs and its further growth. There was no statistical significance in adhesion rates of C albicans to hOMECs. Compared with the control group, the level of hBD-2 mRNA has increased, while hBD-2 and hBD-2 mRNA levels in the rhIL17A + C albicans+ group were the highest. Both hBD-2 and hBD-2 mRNA levels were higher in the rhIL17A+ group than in the C albicans+ group. S100A8 and LL-37 mRNAs have similar trend, and both upregulated after treatment with rhIL17A; however, protein levels were undetectable.Conclusion Recombinant human interleukin 17A may inhibit C albicans from infecting hOMECs by affecting the growth and reproduction of C albicans as well as the formation of hyphae. Besides, rhIL17A might induce hBD-2, S100A8 and LL-37 secretion from hOMECs to strengthen their anti-infective ability.