Fiber types and fiber diameters in canine respiratory muscles.
Fiber types and fiber diameters in canine respiratory muscles.
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犬呼吸肌的纤维类型和纤维直径。
DOI：
10.1152/jappl.1987.62.4.1705
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
JohnsonJr,RL
中科院分区：
文献类型：
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作者：
Reid,MB;Ericson,GC;Feldman,HA;JohnsonJr,RL
在本研究中，我们测量了犬呼吸肌的纤维类型和纤维直径，并检查了膈肌内的区域变化。从八只成年杂种狗身上取出八个膈肌区域、肋间内肌、肋间外肌、腹横肌和肱三头肌的样本，冷冻并进行组织化学处理，用于标准纤维类型和纤维直径测定。呼吸肌由I型和IIa型纤维组成；未发现 IIb 纤维。肌肉之间的纤维成分不同（P 小于 0.0001）。正常 I 型百分比 (+/- SE) 为：膈肌 46 +/- 2、肋间外肌 85 +/- 6、肋间内肌 48 +/- 3、腹横肌 53 +/- 1 和三头肌 33 +/- 7。膈肌还包含 I 型亚型 [6 +/- 1% (SE)]，以前认为仅出现在发育中的肌肉中。隔膜区域之间的纤维成分有所不同（P 小于 0.01）。最值得注意的是，左脚内侧含有 64% 的 I 型纤维。肌肉（P 小于 0.025）和膈肌区域（P 小于 0.0005）之间的纤维尺寸也存在系统性差异。外部肋间纤维直径最大（47-50 微米），隔膜最小（34 微米）。在横膈膜内，脚纤维大于肋纤维（P 小于 0.05）。我们的结论是，犬呼吸肌的纤维成分和纤维直径存在系统差异。
In the present study, we measured fiber types and fiber diameters in canine respiratory muscles and examined regional variation within the diaphragm. Samples of eight diaphragm regions, internal intercostals, external intercostals, transversus abdominis, and triceps brachii were removed from eight adult mongrel dogs, frozen, and histochemically processed for standard fiber type and fiber diameter determinations. The respiratory muscles were composed of types I and IIa fibers; no IIb fibers were identified. Fiber composition differed between muscles (P less than 0.0001). Normal type I percent (+/- SE) were: diaphragm 46 +/- 2, external intercostal 85 +/- 6, internal intercostals 48 +/- 3, transversus abdominis 53 +/- 1, and triceps 33 +/- 7. The diaphragm also contained a type I subtype [6 +/- 1% (SE)] previously thought only to occur in developing muscle. Fiber composition varied between diaphragm regions (P less than 0.01). Most notably, left medial crus contained 64% type I fibers. Fiber size also varied systematically among muscles (P less than 0.025) and diaphragm regions (P less than 0.0005). External intercostal fiber diameter was largest (47–50 microns) and diaphragm was smallest (34 microns). Within diaphragm, crural fibers were larger than costal (P less than 0.05). We conclude that there are systematic differences in fiber composition and fiber diameter of the canine respiratory muscles.