16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts.
16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts.
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用于测量生物工程心脏电生理特性的 16 通道灵活系统。
DOI：
10.1007/s13239-017-0336-8
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发表时间：
2018
影响因子：
1.8
通讯作者：
Birla,RaviK
中科院分区：
文献类型：
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作者：
Salazar,BetsyH;Hoffman,KristopherA;Reddy,AnilkumarK;Madala,Sridhar;Birla,RaviK
随着组织工程学的不断成熟，有必要开发新的技术来洞察当前的范例并指导未来实验的改进。为此，我们开发了一个系统来表征我们的生物人工心脏模型，并将它们与功能天然结构进行比较。在本研究中，成年Spraogue-Dawley的心脏被脱细胞，形成了一个天然的三维心脏支架。新生大鼠原代心肌细胞在复合3D纤维蛋白凝胶中培养，形成三维心脏结构，将其缝合到无细胞支架上并悬浮在介质中24-48小时，然后在不同构型的16个电极上粘贴生物人工心脏(BAHS)，不仅评估培养组织的心电图特征，还测试系统的一致性。组织学检查示心肌细胞化及心肌组织形成。然后，使用我们的16通道灵活系统对BAHS和自然心脏进行评估，以获得与它们各自的电生理特性相关的指标。本地信号之间的时间延迟在0-95毫秒的范围内。此外，彩色图谱还显示了脉冲在整个土著心脏中传播的趋势。在对正常大鼠QRS波群进行评价后，我们发现R波的平均幅度为5351.48±44.92μV，QRS波的平均时程为10.61ms±0.18ms。相比之下，BaHs表现出更不稳定和不均匀的活动，没有得到明显的量化。本研究收集的数据证明了本系统在EKG数据获取中的有效性。
As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague–Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24–48 h. The resulting bioartificial hearts (BAHs) were then affixed with 16 electrodes, in different configurations to evaluate not only the electrocardiographic characteristics of the cultured tissues, but to also test the system’s consistency. Histological evaluation showed cellularization and cardiac tissue formation. The BAHs and native hearts were then evaluated with our 16-channel flexible system to acquire the metrics associated with their respective electrophysiological properties. Time delays between the native signals were in the range of 0–95 ms. As well, color maps revealed a trend in impulse propagation throughout the native hearts. After evaluation of the normal rat QRS complex we found the average amplitude of the R-wave to be 5351.48 ± 44.92μV and the average QRS duration was found to be 10.61 ± 0.18 ms. In contrast, BAHs exhibited more erratic and non-uniform activity that garnered no appreciable quantification. The data collected in this study proves our system’s efficacy for EKG data procurement.