Comparative distribution patterns of type I and III collagens and fibronectin in human arteriosclerotic aorta.
Comparative distribution patterns of type I and III collagens and fibronectin in human arteriosclerotic aorta.
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人动脉硬化主动脉中 I 型和 III 型胶原蛋白和纤连蛋白的分布模式比较。
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发表时间：
1985
期刊：
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通讯作者：
L. Robert
中科院分区：
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作者：
J. Labat;M. Szendrői;G. Godeau;L. Robert
本文研究了人动脉粥样硬化病变和同一动脉粥样硬化无任何肉眼可见病变的区域中I型和III型胶原和纤连蛋白的分布模式。通过间接免疫荧光或免疫过氧化物酶分析，用在兔中制备的抗人血浆纤连蛋白和抗人I型和III型胶原的特异性抗血清，分析冷冻切片的纤连蛋白和胶原。根据Junqueira等人（1979）描述的方法，使用Picro Sirius red对组织样本进行I型和III型胶原分布染色。在没有任何可见肉眼病变的区域，I型和III型胶原在主动脉壁的介质中共分布，其中I型胶原占优势。I型胶原蛋白在内膜中也是可见的，作为连续层。这是由于主动脉的年龄依赖性变化（动脉硬化）。纤连蛋白可在内皮下显示为薄层，在中膜上显示为弱免疫荧光。在近期或进展性动脉粥样硬化斑块中，纤连蛋白和III型胶原蛋白似乎强烈增加和叠加。在斑块的内膜区域中也存在I型胶原蛋白的增加。III型胶原和纤连蛋白在动脉粥样硬化斑块中的共同分布可能表明组织修复类型的过程，类似于在伤口愈合期间可以观察到的过程。我们的研究结果表明，在动脉病变，III型胶原和纤维连接蛋白主要是合成。（250字处删节）
The distribution patterns of type I and III collagens and fibronectin were studied in arteriosclerotic lesions of human aortas as well as in the regions devoid of any macroscopic lesions of the same aortas. Frozen sections were analyzed for fibronectin and collagens by indirect immunofluorescence or immunoperoxidase analysis with specific antisera prepared in rabbits against human plasma fibronectin and against human type I and III collagens. Tissue samples were also stained for type I and III collagen distribution using Picro Sirius red according to the method described by Junqueira et al. (1979). In the regions devoid of any visible macroscopic lesions, there is a co-distribution in the media of the aortic wall of type I and III collagens, with a predominance of type I collagen. Type I collagen is also visible in the intima, as a continuous layer. This is attributed to an age-dependent change of the aorta (arteriosclerosis). Fibronectin can be demonstrated as a thin layer in the subendothelium and as a weak immunofluorescence over the media. In recent or evolutive arteriosclerotic plaques, fibronectin and type III collagen appear to be strongly increased and superimposed. There is also an increase of type I collagen in the intimal region of the plaque. The co-distribution of type III collagen and fibronectin in the arteriosclerotic plaques may suggest a tissue-repair-type of process, analogous to that which can be observed during wound healing. Our results suggest that in arteriosclerotic lesions, type III collagen and fibronectin are predominantly synthesized.(ABSTRACT TRUNCATED AT 250 WORDS)