Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa
Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa
复制标题
来自 Tegillarca granosa 的新型血红蛋白衍生突变肽 (mTgHbP7) 的预测和表征
DOI：
10.1016/j.fsi.2022.05.007
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发表时间：
2022
影响因子：
4.7
通讯作者：
Yongbo Bao
中科院分区：
文献类型：
--
作者：
Xiaoying Zheng;Chun Yuan;Yang Zhang;Shanjie Zha;Fan Mao;Yongbo Bao
从泥鳅中鉴定出一种血红蛋白(Hb)，其衍生的多肽已被证明对革兰氏阳性和革兰氏阴性细菌具有抗菌活性。在本研究中，我们发现了一系列新的抗菌肽和人工诱变的抗菌肽，其中一种突变的抗菌肽(m-Tg-HBP)m-Tg-HbP7被证明对三种细菌(溶藻弧菌、副溶血弧菌和大肠杆菌)具有明显的抗菌活性。此外，预测m TG HbP7形成α-螺旋结构，这是已知的杀菌AMPS的重要特征。此外，通过与HEK293细胞的接触，我们证实了m-TG-HbP7在高浓度(160μ-M)时对哺乳动物细胞没有细胞毒性。因此，本研究结果为软体动物血红蛋白抗菌肽的预测和优化提供了依据，为今后抗菌药物的开发奠定了基础。·鉴定了一系列来源于泥鳅血红蛋白的抗菌肽(Tg HbP1-6)。·m-Tg-HbP7是通过对Tg-HbP2进行人工突变而获得的，具有良好的抗菌效果。·m TG HbP7对哺乳动物细胞无细胞毒性。
The hemoglobin (Hb) is identified in Tegillarca granosa and its derived peptides have been proved to possess antibacterial activity against gram-positive and gram-negative bacteria. In this study, we identified a series of novel antimicrobial peptides (AMPs) and artificially mutated AMPs derived from subunits of T. granosa Hbs, among which, a mutant T. granosa hemoglobin peptide (m Tg HbP) m Tg HbP7, was proved to possess predominant antibacterial activity against three bacteria strains ( Vibrio alginolyticus , V. parahaemolyticus and Escherichia coli ). Besides, m Tg HbP7 was predicted to form α-helical structure, which was known to be an important feature of bactericidal AMPs. Furthermore, upon contact with HEK293 cell line, we confirmed that m Tg HbP7 had no cytotoxicity to mammalian cell even at a high concentration of 160 μM. Therefore, the findings reported here provide a rationalization for antimicrobial peptide prediction and optimization from mollusk hemoglobin, which will be useful for future development of antimicrobial agents. • A series of antimicrobial peptides ( Tg HbP1-6) derived from hemoglobin in Tegillarca granosa were identified. • m Tg HbP7 was obtained by artificially mutation of Tg HbP2, possessing superior antibacterial effect. • m Tg HbP7 exerted no cytotoxicity to mammalian cell.