Differential expression and function of CD80 (B7‐1) and CD86 (B7‐2) on human peripheral blood monocytes
Differential expression and function of CD80 (B7‐1) and CD86 (B7‐2) on human peripheral blood monocytes
复制标题
人外周血单核细胞CD80(B7-1)和CD86(B7-2)的差异表达及功能
作者：
J. Fleischer;E. Soeth;N. Reiling;E. Grage‐Griebenow;H. Flad;Martin Ernst
CD28与其配体的相互作用对于T细胞的激活是重要的。最近的研究表明，在辅助细胞上至少存在两种配体，CD80 （B7‐1）和CD86 （B7‐2）。在这项研究中，我们证明，尽管CD80和CD86都在单核细胞上表达，但它们似乎具有不同的功能。新分离的单核细胞表达CD86，但CD80阴性。CD80的表达在体外培养6-8小时后弱诱导，刺激后表达增强。CD86的表达比CD80的表达增强更快，并在刺激后4-6小时达到峰值水平。逆转录聚合酶链反应研究表明，新分离的单核细胞不含CD80‐mRNA。培养4-6小时后，CD80 mRNA被诱导表达，与CD80蛋白的表达相匹配。使用不同抗体对这两种分子和融合蛋白CTLA4Ig的抑制研究表明，只有抗CD80和CTLA4Ig可以部分抑制抗原特异性（结核菌素）和多克隆（抗CD3）淋巴细胞的增殖和干扰素γ (IFN γ)的分泌。IFN‐γ分泌对阻断共刺激比增殖更敏感。抗体BB‐1没有抑制增殖和细胞因子分泌，抗CD86克隆IT2.2也没有。CTLA4Ig结合CD80和CD86，与抗CD80抗体具有相同的抑制能力。根据这些发现，我们得出结论，人类单核细胞使用CD80作为CD28的共刺激配体，并利用B7家族分子介导的其他共刺激机制。
The interaction of CD28 with its ligands is important for T‐cell activation. Recent studies demonstrated the existence of at least two ligands on accessory cells, CD80 (B7‐1) and CD86 (B7‐2). In this study we demonstrate that, although CD80 and CD86 are both expressed on monocytes, they seem to have different functions. Freshly isolated monocytes express CD86 but are CD80‐negative. CD80 expression is weakly induced after 6–8 hr of in vitro culture and is enhanced by stimulation. CD86 expression is enhanced faster than CD80 expression and reaches the peak level after 4–6 hr in stimulated cells. Reverse transcription–polymerase chain reaction studies demonstrate that freshly isolated monocytes contain no CD80‐mRNA. The mRNA of CD80 is induced after 4–6 hr of culture, which matches with the expression of the protein. Inhibition studies using different antibodies against both molecules and the fusion protein CTLA4Ig show that only anti‐CD80 and CTLA4Ig could partially inhibit antigen‐specific (tuberculin) and polyclonal (anti‐CD3) lymphoproliferation and interferon‐γ (IFN‐γ) secretion of T cells cocultured with autologous monocytes. IFN‐γ secretion was more sensitive to blocking costimulation than proliferation. The antibody BB‐1 did not inhibit proliferation and cytokine secretion, nor did the anti‐CD86 clone IT2.2. CTLA4Ig, which binds both CD80 and CD86, has the same inhibitory capacity as the anti‐CD80 antibody tested. From those findings we conclude that human monocytes use CD80 as a costimulatory ligand for CD28 and utilize other costimulatory mechanisms besides those mediated via molecules of the B7 family.
CD28 配体、BB-1 和 B7 在体外角质形成细胞和体内银屑病细胞上的表达不一致。
DOI：
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发表时间：
1993
期刊：
The American journal of pathology
影响因子：
--
作者：
Nickoloff,BJ;Mitra,RS;Lee,K;Turka,LA;Green,J;Thompson,C;Shimizu,Y
通讯作者：
Shimizu,Y
影响因子：
4.4
作者：
R. Mitra;T. Judge;F. Nestle;L. Turka;B. Nickoloff
通讯作者：
R. Mitra;T. Judge;F. Nestle;L. Turka;B. Nickoloff
单核细胞向 T 细胞提供一种新的共刺激信号，该信号不是由 CD28/B7 相互作用介导的。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Johnson,JG;Jenkins,MK
通讯作者：
Jenkins,MK