Electrophoresis of polar fluorescent tracers through the nerve sheath labels neuronal populations for anatomical and functional imaging.
Electrophoresis of polar fluorescent tracers through the nerve sheath labels neuronal populations for anatomical and functional imaging.
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DOI：
10.1038/srep40433
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发表时间：
2017-01-13
影响因子：
4.6
通讯作者：
Hedwig B
中科院分区：
文献类型：
--
作者：
Isaacson MD;Hedwig B
将示踪剂输送到神经元群中对于可视化其解剖结构和分析其功能至关重要。在一些模型系统中，荧光蛋白的基因靶向表达是选择的方法；然而，这些遗传工具并不适用于大多数生物体，替代标记方法也非常有限。在这里，我们描述了一种新的神经元标记方法，通过直接通过昆虫神经和神经节的神经鞘的吸电极电泳染料输送。极性示踪分子在不破坏蝗虫听神经功能的情况下进入听神经，同时染色周围感觉结构和中央轴突投影。局部神经元群体可以直接通过大脑表面进行标记，并且通过钙指示剂的电泳递送实现声诱发活动的体内光学成像。该方法为研究外周和中枢感觉通路如何处理刺激提供了新的工具，是研究非模式生物神经系统的重要进展。
The delivery of tracers into populations of neurons is essential to visualize their anatomy and analyze their function. In some model systems genetically-targeted expression of fluorescent proteins is the method of choice; however, these genetic tools are not available for most organisms and alternative labeling methods are very limited. Here we describe a new method for neuronal labelling by electrophoretic dye delivery from a suction electrode directly through the neuronal sheath of nerves and ganglia in insects. Polar tracer molecules were delivered into the locust auditory nerve without destroying its function, simultaneously staining peripheral sensory structures and central axonal projections. Local neuron populations could be labelled directly through the surface of the brain, and in-vivo optical imaging of sound-evoked activity was achieved through the electrophoretic delivery of calcium indicators. The method provides a new tool for studying how stimuli are processed in peripheral and central sensory pathways and is a significant advance for the study of nervous systems in non-model organisms.