Transplantation of porcine hepatocytes cultured with polylactic Acid-o-carboxymethylated chitosan nanoparticles promotes liver regeneration in acute liver failure rats.
Transplantation of porcine hepatocytes cultured with polylactic Acid-o-carboxymethylated chitosan nanoparticles promotes liver regeneration in acute liver failure rats.
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DOI：
10.1155/2011/797503
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发表时间：
2011
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen Z;Chang R;Guan W;Cai H;Tang F;Zhu W;Chen J
本研究将游离猪肝细胞悬液（A组）、胶原凝胶包埋猪肝细胞（B组）、PLA-O-CMC纳米颗粒培养并包埋胶原凝胶的猪肝细胞（C组）和单独的PLA-O-CMC纳米颗粒（D组）分别移植到ALF大鼠腹腔内。结果显示，移植后血浆HGF水平升高，并在12小时达到峰值。 C组大鼠在移植后2、6、12、24和36小时表现出最高血浆HGF水平，在48小时表现出最低HGF水平。血浆 VEGF 水平在移植后 48 小时升高，并在 72 小时达到峰值。 C组大鼠在移植后48小时、72小时和96小时时血浆HGF水平最高。 C组肝功能恢复最快。与 B 组相比，C 组在 HTx 后第 1 天的肝脏基尔 67 抗原标记指数 (Ki-67 LI) 显着升高 (P < .05)。在 HTx 后第 5 天和第 7 天，B 组和 C 组的 Ki-67 LI 高于 A 组和 D 组。总之，将PLA-O-CMC纳米粒子培养并包埋在胶原凝胶中的猪肝细胞腹腔移植可以显着促进ALF大鼠的肝脏再生。
In this study, free porcine hepatocytes suspension (Group A), porcine hepatocytes embedded in collagen gel (Group B), porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel (Group C), and PLA-O-CMC nanoparticles alone (Group D) were transplanted into peritoneal cavity of ALF rats, respectively. The result showed that plasma HGF levels were elevated post-transplantation with a peak at 12 hr. The rats in Group C showed highest plasma HGF levels at 2, 6, 12, 24 and 36 hr post-transplantation and lowest HGF level at 48 hr. Plasma VEGF levels were elevated at 48 hr post-transplantation with a peak at 72 hr. The rats in Group C showed highest plasma HGF levels at 48, 72, and 96 hr post-transplantation. The liver functions in Group C were recovered most rapidly. Compared with Group B, Group C had significant high liver Kiel 67 antigen labeling index (Ki-67 LI) at day 1 post-HTx (P < .05). Ki-67 LI in groups B and C was higher than that in groups A and D at days 5 and 7 post-HTx. In conclusion, intraperitoneal transplantation of porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel can promote significantly liver regeneration in ALF rats.