n-Alkyl glucopyranosides completely inhibit ultrasound-induced cytolysis.
n-Alkyl glucopyranosides completely inhibit ultrasound-induced cytolysis.
复制标题
正烷基吡喃葡萄糖苷完全抑制超声诱导的细胞溶解。
DOI：
10.1016/j.freeradbiomed.2005.07.020
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发表时间：
2005
影响因子：
7.4
通讯作者：
Mitchell,JamesB
中科院分区：
文献类型：
--
作者：
Sostaric,JoeZ;Miyoshi,Norio;Riesz,Peter;DeGraff,WilliamG;Mitchell,JamesB
当细胞暴露于超声波时，观察到的细胞突然溶解的机制可能是机械的和/或自由基的性质。自由基反应发生在声空化气泡的核心和界面区域。由于已知环糖具有抑制自由基链反应的作用，我们研究了不同疏水性的n-烷基-β-d-葡萄糖吡喃苷对体外超声（1.057 MHz）诱导HL-60细胞溶解的影响。含有己基-（5毫米）、庚基-（3毫米）或辛基-（2毫米）正烷基链的正烷基葡萄糖苷在一系列条件下保护100%的细胞群免受超声诱导的细胞溶解，在不含葡萄糖苷的情况下，细胞溶解率为35%至100%。受保护的细胞群也具有长期的生殖能力。然而，亲水性甲基-β-d-glucopyranoside不能保护细胞，即使达到30 mM的浓度。此外，没有一种glucopyranoside可以阻止细胞在机械诱导的剪切应力下的细胞溶解。自旋俘获和电子自旋共振实验证实了在表面活性剂存在和不存在的情况下，细胞悬浮液中存在惯性空化现象。结果表明，表面活性葡萄糖苷能有效地抑制空化气泡崩塌时气/液界面上的细胞毒性自由基和/或其前体。
The mechanism(s) responsible for sudden cytolysis observed when cells are exposed to ultrasound could be mechanical and/or free radical in nature. Free radical reactions are initiated in the core and in the interfacial regions of collapsing acoustic cavitation bubbles. Because cyclic sugars are known to inhibit free radical chain reactions, we investigated the effects of n-alkyl-β-d-glucopyranosides of varying hydrophobicity on ultrasound (1.057 MHz)-induced cytolysis of HL-60 cells in vitro. n-Alkyl glucopyranosides with hexyl- (5 mM), heptyl- (3 mM), or octyl- (2 mM) n-alkyl chains protected 100% of the cell population from ultrasound-induced cytolysis under a range of conditions that resulted in 35 to 100% cytolysis in the absence of glucopyranosides. The protected cell populations also possessed long-term reproductive viability. However, the hydrophilic methyl-β-d-glucopyranoside could not protect cells, even up to a concentration of 30 mM. Furthermore, none of the glucopyranosides could prevent cytolysis of cells from a mechanically induced shear stress. Spin trapping and electron spin resonance experiments confirmed the presence of inertial cavitation in cell suspensions both in the presence and in the absence of the surfactants. It is concluded that surface-active glucopyranosides efficiently quench cytotoxic radicals and/or their precursors at the gas/solution interface of collapsing cavitation bubbles.