Dynamic changes in developmental gene expression in the basal chordate Ciona intestinalis
Dynamic changes in developmental gene expression in the basal chordate Ciona intestinalis
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DOI：
10.1111/j.1440-169x.2005.00794.x
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发表时间：
2005-04
期刊：
影响因子：
4.6
通讯作者：
T. Kawashima;Y. Satou;S. D. Murakami;N. Satoh
中科院分区：
文献类型：
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作者：
T. Kawashima;Y. Satou;S. D. Murakami;N. Satoh
大规模表达序列标签（EST）分析被用来证明在其发展过程中的基础脊索动物玻璃海鞘的全球基因表达谱的动态变化。受精卵被发现储存了大量的母体转录本，随着发育的进行，生物体表达的基因库逐渐变小。此外，在变态过程中，观察到参与胚胎发生的基因的显著部分被下调，此时成虫似乎利用一组不同的基因来形成其身体。至少有25%的参与发育的基因被发现被多次使用。这类信息对于形成对基本脊索动物体形成的总体表达控制计划的全面理解至关重要。
Large‐scale expressed sequence tags (EST) analysis was used to demonstrate a number of dynamic changes in the global gene expression profile of the basal chordate Ciona intestinalis over the course of its development. The fertilized egg was found to store a great variety of maternal transcripts and, as development proceeds, the organism expresses a progressively smaller repertoire of genes. In addition, a significant portion of genes involved in embryogenesis were observed to be downregulated during metamorphosis, at which point the adult appears to utilize a different set of genes to form its body. At least 25% of the genes involved in development were found to be used multiple times. This kind of information is essential to form a comprehensive understanding of the overarching expression‐control plan by which the basic chordate body is formed.