Discrepant effects of culture conditions on survival and function of dopaminergic neurons.
Discrepant effects of culture conditions on survival and function of dopaminergic neurons.
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培养条件对多巴胺能神经元的存活和功能的不同影响。
DOI：
10.1097/00001756-200404290-00018
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发表时间：
2004
期刊：
影响因子：
1.7
通讯作者：
Friedmann,Theodore
中科院分区：
文献类型：
--
作者：
Smith,DougW;Friedmann,Theodore
中脑原代培养对于探索多巴胺能神经元的功能和阐明导致其功能障碍和变性的因素是有价值的。为了更有效地控制培养环境，我们对多巴胺能神经元在无血清情况下的存活、分化和营养因子反应进行了表征。介质和补充剂的组合对测量的所有三个指数都有显著影响。产生最大多巴胺能神经元存活的组合不同于那些导致最大分化和营养因子反应的组合。此外，只有一种培养液/补充剂组合的抗氧化剂处理是有效的，这表明在本研究中使用的大多数培养条件下，这些神经元没有由于氧化应激而退化。这些结果表明，多巴胺能神经元可以在无血清条件下生长，但培养条件的选择对细胞的存活和功能有显着影响。
Primary midbrain cultures are valuable for probing the function of dopaminergic neurons and for elucidating the factors that cause their dysfunction and degeneration. To allow more effective control of the culture environment, we have characterized the survival, differentiation, and trophic factor response of dopaminergic neurons in the absence of serum. Combinations of media and supplements markedly affected all three indices measured. Combinations that produced maximal dopaminergic neuron survival are different from those that result in maximal differentiation and trophic factor response. Furthermore, antioxidant treatment was effective with only one medium/supplement combination, indicating that these neurons were not degenerating as a result of oxidative stress in the majority of culture conditions used in this study. These results demonstrate that dopaminergic neurons can be grown in serum-free conditions but that the choice of culture conditions has a marked influence on cell survival and function.