The chloroplast genome of mulberry: complete nucleotide sequence, gene organization and comparative analysis
The chloroplast genome of mulberry: complete nucleotide sequence, gene organization and comparative analysis
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DOI：
10.1007/s11295-006-0051-3
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Khurana, Paramjit
中科院分区：
文献类型：
--
作者：
Ravi, V.;Khurana, Jitendra P.;Khurana, Paramjit
报道了桑树(Morus inca cv.)K2)叶绿体基因组(158,484bp)已经用长的聚合酶链式反应和基于鸟枪的方法相结合的方法进行了测定。这是第三种被子植物，其质体序列已被完全破译。环状双链分子由两个相同的反向重复序列(每个25,678个核苷酸)组成，分别分隔87,386个核苷酸和19,742个核苷酸的大和小单复制区。根据与其他叶绿体基因组预测基因的同源性，共分配了83个蛋白质编码基因，其中包括5个在反向重复区重复的基因，8个核糖体RNA基因和37个tRNA基因(30个基因种类)，代表20个氨基酸。桑叶质体缺失infA、spA和rp121基因，含有两个假基因ycf15和ycf68。基于序列相似性在基因和基因组水平上的比较分析表明，桑属在系统发育上与黄瓜和莲花更接近。然而，在基因组水平上，包括非编码区使其更接近桉树，紧随其后的是黄瓜。这可能反映了叶绿体基因组基因和基因间隔区的不同选择压力。
The complete nucleotide sequence of mulberry (Morus indica cv. K2) chloroplast genome (15 8,484 bp) has been determined using a combination of long PCR and shotgun-based approaches. This is the third angiosperm tree species whose plastome sequence has been completely deciphered. The circular double-stranded molecule comprises of two identical inverted repeats (25,678 bp each) separating a large and a small single-copy region of 87,386 bp and 19,742 bp, respectively. A total of 83 protein-coding genes including five genes duplicated in the inverted repeat regions, eight ribosomal RNA genes and 37 tRNA genes (30 gene species) representing 20 amino acids, were assigned on the basis of homology to predicted genes from other chloroplast genomes. The mulberry plastome lacks the genes infA, sprA, and rp121 and contains two pseudogenes ycf15 and ycf68. Comparative analysis, based on sequence similarity, both at the gene and genome level, indicates Morus to be closer to Cucumis and Lotus, phylogenetically. However, at genome level, inclusion of non-coding regions brings it closer to Eucalyptus, followed by Cucumis. This may reflect differential selection pressure operating on the genic and intergenic regions of the chloroplast genome.