The trk family of receptors mediates nerve growth factor and neurotrophin-3 effects in melanocytes.
The trk family of receptors mediates nerve growth factor and neurotrophin-3 effects in melanocytes.
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trk 受体家族介导黑素细胞中神经生长因子和神经营养素 3 的作用。
DOI：
10.1172/jci117496
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Gilchrest,BA
中科院分区：
文献类型：
--
作者：
Yaar,M;Eller,MS;DiBenedetto,P;Reenstra,WR;Zhai,S;McQuaid,T;Archambault,M;Gilchrest,BA
我们最近表明(a)人黑色素细胞在体外表达p75神经生长因子（NGF）受体；(b)除其他行为外，黑素细胞树突和迁移至少部分受NGF调节；(c)培养的人表皮角质形成细胞产生NGF。我们现在报道用12-四癸酸13-醋酸酯（TPA）刺激黑素细胞，之前报道过可以诱导p75 NGF受体，也可以诱导黑素细胞中的trk，并且TPA的作用在培养的角质形成细胞中进一步增强。此外，在NGF刺激后的几分钟内，黑素细胞中的trk被磷酸化。同样，真皮成纤维细胞的培养表达神经营养因子-3 (NT-3) mRNA；培养成纤维细胞中NT-3 mRNA水平可通过有丝分裂刺激、紫外线照射和暴露于黑素细胞条件培养基中进行调节。此外，黑素细胞组成性地表达低水平的trk-C，其表达在TPA刺激后下调。单独向培养基199中培养的黑色素细胞补充NT-3可防止细胞死亡。这些综合数据表明，人类皮肤中的黑素细胞行为可能受到神经营养因子的影响，这些因子可能来自角化细胞和成纤维细胞，它们通过高亲和力受体起作用。图片
We have recently shown that (a) human melanocytes express the p75 nerve growth factor (NGF) receptor in vitro; (b) that melanocyte dendricity and migration, among other behaviors, are regulated at least in part by NGF; and (c) that cultured human epidermal keratinocytes produce NGF. We now report that melanocyte stimulation with phorbol 12-tetra decanoate 13-acetate (TPA), previously reported to induce p75 NGF receptor, also induces trk in melanocytes, and TPA effect is further potentiated by the presence of keratinocytes in culture. Moreover, trk in melanocytes becomes phosphorylated within minutes after NGF stimulation. As well, cultures of dermal fibroblasts express neurotrophin-3 (NT-3) mRNA; NT-3 mRNA levels in cultured fibroblasts are modulated by mitogenic stimulation, UV irradiation, and exposure to melanocyte-conditioned medium. Moreover, melanocytes constitutively express low levels of trk-C, and its expression is downregulated after TPA stimulation. NT-3 supplementation to cultured melanocytes maintained in Medium 199 alone prevents cell death. These combined data suggest that melanocyte behavior in human skin may be influenced by neurotrophic factors, possibly of keratinocyte and fibroblast origin, which act through high affinity receptors.Images