Mullerian inhibiting substance suppresses proliferation and induces apoptosis and autophagy in endometriosis cells in vitro.
Mullerian inhibiting substance suppresses proliferation and induces apoptosis and autophagy in endometriosis cells in vitro.
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DOI：
10.1155/2013/361489
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kilic GS
中科院分区：
文献类型：
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作者：
Borahay MA;Lu F;Ozpolat B;Tekedereli I;Gurates B;Karipcin S;Kilic GS
Objective.目的通过研究苗勒管抑制物质（MIS）对子宫内膜异位症细胞凋亡和自噬的影响。设计体外实验研究。设置.大学研究实验室。细胞系CRL-7566子宫内膜异位症细胞系。这条线是从子宫内膜异位症患者的良性卵巢囊肿中建立的。干预。用MIS进行体外治疗。主要结局指标。主要的结果指标是细胞活力，增殖，细胞周期阻滞，以及诱导凋亡和自噬的增生细胞。结果MIS处理抑制子宫内膜异位症细胞的增殖和诱导凋亡，如Annexin V染色所示，并诱导caspase-9裂解和细胞周期停滞，如p27 CDK抑制剂表达增加所证明的。MIS处理还诱导子宫内膜异位症细胞中的自噬，如通过LC 3-II诱导（自噬的标志）的显著增加所证明的。结论. MIS抑制异位子宫内膜细胞系的细胞生长并诱导自噬和凋亡。我们的研究结果表明，MIS可能有潜力作为一种新的药物治疗子宫内膜异位症的方法。可能需要进一步的研究来测试MIS治疗在动物模型中的疗效，并开发专门针对子宫内膜异位症的MIS治疗。
Objective. To determine the effects of Mullerian inhibiting substance (MIS) treatment on endometriosis cells through study of apoptosis and autophagy. Design. Experimental in vitro study. Setting. University research laboratory. Cell Line. CRL-7566 endometriosis cell line. This line was established from a benign ovarian cyst taken from a patient with endometriosis. Interventions. In vitro treatment with MIS. Main Outcome Measures. The main outcome measures were cellular viability, proliferation, cell-cycle arrest, and induction of apoptosis and autophagy in endometriotic cells. Results. MIS treatment inhibited proliferation of endometriosis cells and induced apoptosis, as indicated by Annexin V staining, and induced caspase-9 cleavage and cell-cycle arrest, as evidenced by increased expression of p27 CDK-inhibitor. MIS treatment also induced autophagy in endometriosis cells as demonstrated by a significant increase in LC3-II induction, a hallmark of autophagy. Conclusions. MIS inhibits cell growth and induces autophagy, as well as apoptosis, in ectopic endometrial cell lines. Our results suggest that MIS may have a potential as a novel approach for medical treatment of endometriosis. Further studies may be needed to test the efficacy of MIS treatment in animal models and to develop MIS treatment specifically targeted to the endometriosis.