Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces
Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces
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DOI：
10.1002/jbm.a.31618
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发表时间：
2008-07-01
影响因子：
4.9
通讯作者：
Chung, Hyun-Ju
中科院分区：
文献类型：
--
作者：
Park, Jin-Woo;Suh, Jo-Young;Chung, Hyun-Ju
研究了钙离子（Ca）掺入的钛（Ti）表面的表面特性及其对成骨细胞分化的影响。MC 3 T3-E1前成骨细胞在有和没有钙掺入的机械加工或喷砂钛表面上培养。MTT法检测细胞增殖情况，实时荧光定量PCR法检测成骨细胞基因表达。用含Ca溶液进行水热处理产生了尺寸约为100 nm的结晶CaTiO（3）纳米结构，保留了由机械加工、喷砂或喷砂和蚀刻处理引起的原始微米级表面形貌和微观粗糙度。在Hank平衡盐溶液中浸泡后，在Ca掺入样品的所有表面上观察到相当大的磷灰石形成。在掺入Ca的Ti表面上发现比未处理的Ti表面上显著更多的细胞增殖（p < 0.001）。实时定量PCR分析显示，在早期时间点，在钙掺入喷砂表面上生长的细胞中碱性磷酸酶、骨桥蛋白和骨钙素mRNA水平明显高于其他表面。因此，Ca掺入可能通过在植入后的早期愈合阶段加速成骨细胞增殖和分化对微结构钛植入物的骨整合产生有益影响。(C)2007 Wiley Periodicals，Inc.
The surface characteristics of a calcium ion (Ca)-incorporated titanium (Ti) surface, produced by hydrothermal treatment using an alkaline Ca-containing solution, and its effects on osteoblastic differentiation were investigated. MC3T3-E1 pre-osteoblastic cells were cultured on machined or grit-blasted Ti surfaces with and without Ca incorporation. The MTT assay was used to determine cell proliferation, and real-time PCR was used for quantitative analysis of osteoblastic gene expression. Hydrothermal treatment with a Ca-containing solution produced a crystalline CaTiO(3) nanostructure of approximately 100 nm in dimension, preserving original micron-scaled surface topographies and microroughness caused by machining, blasting, or blasting and etching treatments. After immersion in Hank's balanced salt solution, considerable apatite formation was observed on all surfaces of the Ca-incorporated samples. Significantly more cell proliferation was found on Ca-incorporated Ti surfaces than on untreated Ti surfaces (p < 0.001). Quantitative real-time PCR analysis showed notably higher alkaline phosphatase, osteopontin, and osteocalcin mRNA levels in cells grown on Ca-incorporated blasted surfaces than on other surfaces at an early time point. Thus, Ca incorporation may have a beneficial effect on osseointegration of microstructured Ti implants by accelerating osteoblast proliferation and differentiation during the early healing phase following implantation. (C) 2007 Wiley Periodicals, Inc.