In Vitro Antibacterial and Antitumor Activity of Total Triterpenoids from a Medicinal Mushroom Sanghuangporus sanghuang (Agaricomycetes) in Liquid Fermentation Culture
In Vitro Antibacterial and Antitumor Activity of Total Triterpenoids from a Medicinal Mushroom Sanghuangporus sanghuang (Agaricomycetes) in Liquid Fermentation Culture
复制标题
液体发酵培养药用菌桑黄菌总三萜的体外抗菌和抗肿瘤活性
DOI：
10.1615/intjmedmushrooms.2021038916
复制
发表时间：
2021-01-01
影响因子：
1.2
通讯作者：
He, Xiang Wei
中科院分区：
文献类型：
--
作者：
Ma, Jie Xin;Cai, Cheng Shan;He, Xiang Wei
对药用真菌桑黄孢菌（Sanghuangporus sanghuang）液体发酵培养物中总三萜类化合物的生物活性和化学成分进行了分析。S.其中桑黄总三萜提取物（STTE）对金黄色葡萄球菌的抑制作用最强，2倍最低抑菌浓度的STTE对4种细菌的生长均有显著影响，并引起明显的核酸渗漏。扫描电子显微镜证实，STTE破坏细菌细胞壁。STTE还显著抑制Caco-2细胞的增殖并破坏其形态。流式细胞仪显示STTE可诱导Caco-2细胞周期阻滞，并诱导早期和晚期凋亡。红外光谱法和气相色谱-质谱法导致检测到多种三萜类化合物（例如，五环三萜类、羊毛脂烷三萜类和反式角鲨烯）以及其它小分子（例如，酯、酸和醛）。我们的研究结果表明，STTE具有抗菌和抗肿瘤活性，并将使新的真菌衍生的抗癌和抗菌化合物的筛选。
We analyzed the biological activity and composition of total triterpenoids extracted from the liquid fermentation culture of the medicinal mushroom Sanghuangporus sanghuang. S. sanghuang total triterpenoid extract (STTE) had the strongest inhibitory effect on Staphylococcus aureus, and STTE at 2-fold the minimum inhibitory concentration had a significant effect on the growth of four types of bacteria and caused marked nucleic acid leakage. Scanning electron microscopy confirmed that STTE destroyed bacterial cell walls. STTE also significantly inhibited the proliferation of Caco-2 cells and disrupted their morphology. Flow cytometry revealed that STTE induced cell cycle arrest and induced early and late apoptosis of Caco-2 cells. Infrared spectroscopy, and gas chromatography-mass spectrometry resulted in the detection of a variety of triterpenoids (e.g., pentacyclic triterpenoid, lanolinane triterpenoid, and trans-squalene) as well as other small molecules (e.g., esters, acids, and aldehydes). Our findings indicate that STTE has antibacterial and antitumor activity and will enable screening of novel fungal-derived anticancer and antibacterial compounds.