DNA Colorimetric Logic Gates Based on Triplex-Helix Molecular Switch
DNA Colorimetric Logic Gates Based on Triplex-Helix Molecular Switch
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基于三螺旋分子开关的DNA比色逻辑门
DOI：
10.1021/jp503608t
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发表时间：
2014-07-03
影响因子：
3.7
通讯作者：
Qiu, Jian-Ding
中科院分区：
文献类型：
--
作者：
Gao, Wan;Zhang, Li;Qiu, Jian-Ding
一个类似辣根过氧化物酶(HRP)的DNAzyme序列首先被基于三链的分子信标(TMB)阻断。当与单链DNA输入杂交时，发生三螺旋分子开关，释放的产物链自组装成氯化血红素/G-四链-HRP模拟DNAzyme，该DNAzyme生物催化形成有色产物，并为不同的逻辑门提供输出信号。基于这一原理，人们开发了一系列的逻辑门(或、异或、抑制、与)。此外，通过连接AND和XOR逻辑门，开发了实施整体OR布尔行为的多级电路(MC)。逻辑输出信号肉眼可识别，为设计复杂的DNA基逻辑器件提供了一种灵活、安全、经济、简单的方法。
A horseradish peroxidase (HRP)-mimicking DNAzyme sequence is first blocked by the triplex-based molecular beacon (tMB). Upon hybridization with single-stranded DNA inputs, triplex-helix molecular switch occurs, and the released product strand self-assembles into the hemin/G-quadruplex-HRP-mimicking DNAzyme that biocatalyzes the formation of a colored product and provides an output signal for the different logic gates. On the basis of this principle, a series of logic gates (OR, XOR, INHIBIT, and AND) have been developed. Moreover, a multilevel circuit (MC) that enforces an overall OR Boolean behavior is developed by connecting the AND and XOR logic gates. The logic output signals can be recognized by naked eyes, thus providing a flexible, secure, economic, and simple method for designing a complex DNA-based logic device.