A method for partial purification of lamellar granules from fetal rat epidermis.
A method for partial purification of lamellar granules from fetal rat epidermis.
复制标题
一种从胎鼠表皮中部分纯化层状颗粒的方法。
DOI：
10.1111/1523-1747.ep12497735
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Traczyk,TN
中科院分区：
文献类型：
--
作者：
Freinkel,RK;Traczyk,TN
通过在甲曲酰胺中进行密度梯度分级，从胎鼠表皮匀浆中获得富含层状颗粒的亚细胞级分。该部分中 62% 的细胞器被单膜包围，测量的较短直径为 80–130 nm。其中大约 10% 具有特征性的片层。该部分中的其他结构是较大的囊泡（20%）或较小的细胞器和囊泡（18%）。该级分的浮力密度较低（1.08-1.10），表明脂质含量较高，并且含有尖锐的酸性磷酸酶活性局部峰。上午 80-130 点的细胞器在用高碘酸氧化后与铋发生反应，并且呈酸性磷酸酶阳性。其他细胞内细胞器（例如溶酶体、线粒体、核糖体）以及角蛋白原纤维和透明角质蛋白不存在。得出的结论是，该部分大部分由层状颗粒组成，从而首次允许对这些细胞器的组成和代谢进行详细研究。
A subcellular fraction enriched with lamellar granules was obtained from homogenates of fetal rat epidermis by means of density gradient fractionation in metrizamide. 62% of organelles in this fraction were bounded by single membranes and measured 80–130 nm in the shorter diameter. About 10% of these had characteristic lamellae. Other structures in this fraction were larger vesicles (20%) or smaller organelles and vesicles (18%). The fraction had a low buoyant density (1.08–1.10) suggesting a high lipid content and contained a sharply localized peak of acid phosphatase activity. The 80–130 am organelles reacted with bismuth after oxidation with periodic acid and were positive for acid phosphatase. Other intracellular organelles (eg., lysosomes, mitochondria, ribosomes) as well as keratin fibrils and keratohyalin were not present.It is concluded that most of this fraction consists of lamellar granules, permitting for the first time detailed investigations of the composition and metabolism of these organelles.