HepG2 Cells Expressing MicroRNA miR-122 Support the Entire Hepatitis C Virus Life Cycle
HepG2 Cells Expressing MicroRNA miR-122 Support the Entire Hepatitis C Virus Life Cycle
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DOI：
10.1128/jvi.05843-11
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发表时间：
2011-11-01
影响因子：
5.4
通讯作者：
Evans, Matthew J.
中科院分区：
文献类型：
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作者：
Narbus, Christopher M.;Israelow, Benjamin;Evans, Matthew J.
肝脏特异性microRNA miR-122是细胞培养和体内有效复制丙型肝炎病毒（HCV）RNA所必需的。此外，通过miR-122表达，非肝细胞在支持HCV生命周期的这一阶段方面更有效。本研究探讨了miR-122如何影响HCV在miR-122缺陷型HepG 2细胞系中的复制。这种microRNA在HepG 2细胞中的表达允许有效的HCV RNA复制和感染性病毒体产生。当缺失的HCV受体也表达时，这些细胞有效地支持病毒进入，从而支持整个HCV生命周期。
The liver-specific microRNA miR-122 is required for efficient hepatitis C virus (HCV) RNA replication both in cell culture and in vivo. In addition, nonhepatic cells have been rendered more efficient at supporting this stage of the HCV life cycle by miR-122 expression. This study investigated how miR-122 influences HCV replication in the miR-122-deficient HepG2 cell line. Expression of this microRNA in HepG2 cells permitted efficient HCV RNA replication and infectious virion production. When a missing HCV receptor is also expressed, these cells efficiently support viral entry and thus the entire HCV life cycle.