Identification and molecular characterization of a new β-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae
Identification and molecular characterization of a new β-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae
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DOI：
10.1007/s10658-012-9997-1
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发表时间：
2012-10-01
影响因子：
1.8
通讯作者：
Peng, Deliang
中科院分区：
文献类型：
--
作者：
Long, Haibo;Peng, Huan;Peng, Deliang
植物寄生线虫咽腺细胞中表达的基因编码的分泌蛋白在线虫对植物的寄生中起着关键作用。从禾谷孢囊线虫Heterodera avenae中分离到一个新的β-1，4-内切葡聚糖酶基因（Ha-eng-1a）。Ha-eng-1a的cDNA编码一个463个氨基酸的序列，包含一个催化结构域和一个纤维素结合模块。Ha-eng-1a基因组DNA长2,129 bp，包含8个内含子和9个外显子，内含子长度在56 ~ 157 bp之间，外显子长度在70 ~ 299 bp之间。Southern杂交分析表明Ha-eng-1a基因有两个拷贝。原位杂交结果表明，Ha-eng-1a转录产物在第二阶段幼鱼的两个亚腹腺细胞中特异性积累。重组蛋白Ha-eng-1a在体外具有纤维素酶活性。结果表明，该β-1，4-内切葡聚糖酶基因可能在线虫侵入和迁移过程中对植物细胞壁的降解起关键作用。
Secretory proteins encoded by genes expressed in the pharyngeal gland cells of plant-parasitic nematodes have key roles in nematode parasitism of plants. A new beta-1,4-endoglucanase gene (designated Ha-eng-1a) was isolated from the cereal cyst nematode Heterodera avenae. The cDNA of Ha-eng-1a encoded a deduced 463-amino acid sequence containing a catalytic domain and a cellulose binding module separated by a linker. The genomic DNA of Ha-eng-1a is 2,129-bp long, containing eight introns ranging from 56 bp to157 bp and nine exons ranging from 70 bp to 299 bp. Southern blot analysis revealed that the Ha-eng-1a gene has two copies. In situ hybridization showed that the Ha-eng-1a transcriptsspecifically accumulated in the two subventral gland cells of the second-stage juveniles. There was evidence for cellulase activity of the recombinant protein Ha-eng-1a in vitro. The results indicated that this beta-1,4-endoglucanase gene may play a crucial role in plant cell wall-degradation during penetration and migration of nematodes in the host roots.