Stem Cell Proliferation Pathways Comparison between Human Exfoliated Deciduous Teeth and Dental Pulp Stem Cells by Gene Expression Profile from Promising Dental Pulp
Stem Cell Proliferation Pathways Comparison between Human Exfoliated Deciduous Teeth and Dental Pulp Stem Cells by Gene Expression Profile from Promising Dental Pulp
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DOI：
10.1016/j.joen.2009.07.024
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发表时间：
2009-11-01
影响因子：
4.2
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
--
作者：
Nakamura, Sayaka;Yamada, Yoichi;Ueda, Minoru
间充质干细胞（MSCs）已被用于组织工程和再生医学（TERM）的临床应用。迄今为止，MSC最常见的来源是骨髓。然而，骨髓穿刺对捐赠者来说是一个侵入性和痛苦的过程。因此，MSC的替代来源的鉴定和表征非常重要。本研究旨在比较人脱落乳牙干细胞（SHED）与牙髓干细胞（DPSC）和骨髓间充质干细胞（BMMSC）的特性。方法：比较DPSCs、SHED、BMMSCs的增殖率、干细胞标志物表达等“干性”。此外，应用基因芯片技术分析DPSCs和SHED的基因表达谱。结果如下：从三个来源分离的所有细胞均表现出MSC特征，包括成纤维细胞形态和间充质干细胞标志物的表达。SHED的增殖率明显高于DPSCs和BMMSCs（P < 0.05）。基因表达谱的比较表明，4386个基因在DPSC和SHED之间的表达变化为2.0倍或更多。在SHED中观察到参与细胞增殖和细胞外基质相关途径的基因表达较高，包括成纤维细胞生长因子和肿瘤生长因子β等几种细胞因子。结论：由于其具有更高的增殖能力、丰富的细胞供应和无痛的干细胞收集以及最小的侵袭的优点，SHED可能是作为潜在治疗应用的细胞来源的理想选择。（J Endod 2009;35：1536-1542）
Introduction: Mesenchymal stem cells (MSCs) have been used for clinical application in tissue engineering and regenerative medicine (TERM). To date, the most common source of MSCs has been bone marrow. However, the bone marrow aspirate is an invasive and painful procedure for the donor. Thus, the identification and characterization of alternative sources of MSCs are of great importance. This study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) compared with dental pulp stem cells (DPSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs). Methods: We have compared "sternness" such as the proliferation rate and the expression of stem cell marker of DPSCs, SHED, and BMMSCs. In addition, gene expression profile of DPSCs and SHED were analyzed by using DNA microarray. Results: All cells isolated from the three sources exhibited MSC characteristics including a fibroblastic morphology, and the expression of mesenchymal stem-cell markers. The proliferation rate of SHED was significantly higher than that of DPSCs and BMMSCs (P < 0.05). The comparison of the gene expression profiles indicated 4386 genes with a changed expression between DPSCs and SHED by 2.0-fold or more. Higher expression in SHED was observed for genes that participate in pathways related to cell proliferation and extracellular matrix, including several cytokines such as fibroblast growth factor and tumor growth factor beta. Conclusions: Because of its advantages of a higher proliferation capability, abundant cell supply, and painless stem cell collection with minimal invasion, SHED could be a desirable option as a cell source for potential therapeutic applications. (J Endod 2009;35:1536-1542)