Involvement of the xyloglucan endotransglycosylase/hydrolases encoded by celery XTH1 and Arabidopsis XTH33 in the phloem response to aphids
Involvement of the xyloglucan endotransglycosylase/hydrolases encoded by celery XTH1 and Arabidopsis XTH33 in the phloem response to aphids
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DOI：
10.1111/j.1365-3040.2006.01618.x
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发表时间：
2007-02-01
影响因子：
7.3
通讯作者：
Dinant, Sylvie
中科院分区：
文献类型：
--
作者：
Divol, Fanchon;Vilaine, Francoise;Dinant, Sylvie
在侵染期间，取食韧皮部的昆虫诱导植物中的转录重编程，这可能导致保护。芹菜XTH 1基因，编码木葡聚糖内转糖基酶/水解酶（XTH）的转录本，先前被发现积累在芹菜（芹菜）韧皮部系统，与通才蚜虫桃蚜侵染。XTH 1的诱导是特定的韧皮部，但不相关的木葡聚糖内糖基转移酶（XET）的活性在韧皮部的增加。XTH 1与拟南芥XTH 33基因同源。在M.桃蚜侵染XTH 33表达的模式在发育过程中受到严格控制，并表明在细胞扩增中可能起作用。用M.桃蚜。蚜虫优先定居在突变体上，而不是野生型。这表明XTH 33参与保护植物免受蚜虫侵害;因此，细胞壁修饰可以改变蚜虫对特定植物的偏好。然而，XTH 33在韧皮部组织中的异位表达不足以提供保护，表明修饰该单一基因的表达不容易改变植物-蚜虫相互作用。
During infestation, phloem-feeding insects induce transcriptional reprogramming in plants that may lead to protection. Transcripts of the celery XTH1 gene, encoding a xyloglucan endotransglycosylase/hydrolase (XTH), were previously found to accumulate systemically in celery (Apium graveolens) phloem, following infestation with the generalist aphid Myzus persicae. XTH1 induction was specific to the phloem but was not correlated with an increase in xyloglucan endotransglycosylase (XET) activity in the phloem. XTH1 is homologous to the Arabidopsis thaliana XTH33 gene. XTH33 expression was investigated following M. persicae infestation. The pattern of XTH33 expression is tightly controlled during development and indicates a possible role in cell expansion. An xth33 mutant was assayed for preference assay with M. persicae. Aphids settled preferentially on the mutant rather than on the wild type. This suggests that XTH33 is involved in protecting plants against aphids; therefore, that cell wall modification can alter the preference of aphids for a particular plant. Nevertheless, the ectopic expression of XTH33 in phloem tissue was not sufficient to confer protection, demonstrating that modifying the expression of this single gene does not readily alter plant-aphid interactions.