Mechanism of monofunctional and bifunctional alkylation of DNA by mitomycin C.
Mechanism of monofunctional and bifunctional alkylation of DNA by mitomycin C.
复制标题
丝裂霉素 C 对 DNA 进行单功能和双功能烷基化的机制。
DOI：
10.1021/bi00409a009
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Lipman,R
中科院分区：
文献类型：
--
作者：
Tomasz,M;Chawla,AK;Lipman,R
Materials and MethodsMaterials and their sources are as follows: Micrococcus luteus DNA, Worthington Biochemicals (sonicated before use); mitomycin C, Bristol Laboratories, Syracuse, NY; 10-decarbamoyl-MC, synthesized as described (Kinoshita et al., 1970); all other materials, as described previously (Tomasz et al., 1986).Reactions of DNA with MC under Variations in the Re-ductive Activating Conditions and Isolation of the Resulting MC-DNA Complexes.(A) H2/Pt02 as Reducing Agent,(i) Standard reaction: M. luteus DNA (0.67 gmol/mL), MC (0.33 µ/mL), and Pt02 catalyst (100¿^/µ of MC) were mixed in 0.015 MTris-HCl (pH 7.4) and hydrogenated by a published procedure (Tomasz et al., 1983). Isolation and determination of the binding ratio (br, mole of MC per mole of mononucleotide) of the resulting MC-DNA complex were
