Identification of antibodies as biological markers in serum from multiple sclerosis patients by immunoproteomic approach
Identification of antibodies as biological markers in serum from multiple sclerosis patients by immunoproteomic approach
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DOI：
10.1016/j.jneuroim.2010.11.003
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发表时间：
2011-04-01
影响因子：
3.3
通讯作者：
Inuzuka, Takashi
中科院分区：
文献类型：
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作者：
Sakurai, Takeo;Kimura, Akio;Inuzuka, Takashi
我们通过蛋白质组学分析从多发性硬化症(MS)患者的血清中鉴定了抗线粒体热休克蛋白70(MtHSP70)的抗体。MS患者血清中抗mtHSP70抗体的阳性率显著高于帕金森病患者、多发性脑梗塞患者、感染性脑膜脑炎患者和健康对照组(68%的敏感性；74%的特异性)。我们研究了抗mtHSP70抗体阳性的MS患者的临床特征和磁共振成像表现。结果表明，抗mtHSP70抗体阳性与阴性的MS患者之间无显著差异。此外，在我们对抗mtHSP70抗体和抗磷酸甘油变位酶1(PGAM1)抗体在MS患者血清中的患病率进行的综合分析中，MS患者血清中这两种抗体的阳性率显著高于其他神经系统疾病患者和HCS患者的血清中这两种抗体的阳性率；而且，这种联合检测的特异性高于单一抗体检测的特异性(敏感度为57%；特异度为93%)。我们的研究结果表明，不仅抗PGAM1抗体，而且抗mtHSP70抗体都是MS的良好诊断标志，这两种抗体联合使用有助于MS(C)2010 Elsevier B.V.的更特异的诊断。
We identified the antibody against mitochondrial heat shock protein 70 (mtHSP70) in serum from multiple sclerosis (MS) patients by proteomics-based analysis. The prevalence of the anti-mtHSP70 antibody is significantly higher in serum from MS patients than in serum from Parkinson disease patients, multiple cerebral infarction patients, infectious meningoencephalitis patients, and healthy controls (HCs) (68% sensitivity; 74% specificity). We studied the clinical features and magnetic resonance imaging findings of MS patients with the anti-mtHSP70 antibody. As a result, there were no significant differences between the anti-mtHSP70-antibody-positive and -negative MS patients. Additionally, in our comprehensive analysis of the prevalence of both the anti-mtHSP70 antibody and the anti-phosphoglycerate mutase 1 (PGAM1) antibody, which was previously reported by us to also show a higher prevalence in serum from MS patients, the positivity rates of both these antibodies were significantly higher in serum from MS patients than in serum from patients with other neurological diseases and from HCs; moreover, the specificity of this combination assay was higher than that of the assay of only one antibody (57% sensitivity; 93% specificity). Results of our study suggest that not only the anti-PGAM1 antibody but also the anti-mtHSP70 antibody is good diagnostic markers of MS and the combination of both these antibodies is useful for a more specific diagnosis of MS. (C) 2010 Elsevier B.V. All rights reserved.