IMMUNOFLUORESCENCE VISUALIZATION OF GERM-LINE-SPECIFIC CYTOPLASMIC GRANULES IN EMBRYOS, LARVAE, AND ADULTS OF CAENORHABDITIS-ELEGANS
IMMUNOFLUORESCENCE VISUALIZATION OF GERM-LINE-SPECIFIC CYTOPLASMIC GRANULES IN EMBRYOS, LARVAE, AND ADULTS OF CAENORHABDITIS-ELEGANS
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DOI：
10.1073/pnas.79.5.1558
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
WOOD, WB
中科院分区：
文献类型：
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作者：
STROME, S;WOOD, WB
利用荧光抗体染色技术，对生殖细胞特有的胞质颗粒进行了跟踪研究。优美的这些元素，指定的P颗粒，被隔离专门生殖系前体细胞在早期胚胎发生。在产生体细胞和生殖系子细胞的每个早期分裂的有丝分裂之前，颗粒定位于注定用于生殖系子细胞的细胞质区域。在16细胞期后，颗粒似乎与核膜结合。P颗粒在整个幼虫期和成虫期都存在于生殖细胞中。P颗粒在数量、大小和分布上与经EM鉴定为种质的种系特异结构相似。线虫胚胎
By using fluorescent antibody staining, the cytoplasmic granules unique to germ-line cells were followed throughout the life cycle of C. elegans. These elements, designated P granules, are segregated exclusively to germ-line precursor cells during early embryogenesis. Prior to mitosis at each of the early cleavages that produce a somatic and germ-line daughter cell, the granules become localized in the region of cytoplasm destined for the germ-line daughter. After the 16-cell stage, the granules appear to be associated with the nuclear envelope. P granules persit in the germ cells throughout the larval and adult stages. The P granules are similar in number, size and distribution to germ-line-specific structures identified as germinal plasm by EM in C. elegans embryos.