Differential gene expression in Ulva prolifera under low light and low temperature conditions
Differential gene expression in Ulva prolifera under low light and low temperature conditions
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DOI：
10.1007/s00294-012-0380-8
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发表时间：
2012-08-01
期刊：
影响因子：
2.5
通讯作者：
Ye, Naihao
中科院分区：
文献类型：
--
作者：
Li, Youxun;Zhang, Xiaowen;Ye, Naihao
近年来，作为黄海接连发生的大规模绿潮的重要致病物种，海洋绿色大型藻类--增殖石榴受到了全球越来越多的关注。在可能管理这些藻类水华之前，准确定位“种子”来源是需要解决的主要科学问题之一。已有研究表明，生长在寒冷海底沉积物中的增殖石榴体细胞可能是其主要的繁殖体库之一。为了确定这一假说背后的分子机制，本研究采用抑制性消减杂交技术分析了增殖石榴在弱光和低温条件下的差异基因表达(与寒冷的海底沉积物条件相匹配，6摄氏度，30微克分子光子m(-2)S(-1))。在本研究中，共检测到137个EST，分别代表88个非基因区(80个单态和8个重叠群)，而在130个EST中有109个非基因区(96个单态和13个重叠群)表达下调。BLASTX分析表明，65%的高表达基因和59%的下调基因不属于公共数据库中任何已记录的功能注释或假设蛋白质。然而，对功能定义序列的分析表明：(1)明显的衰老迹象；(2)光合作用系统和戊糖磷酸途径的增强；(3)在弱光和低温条件下，石榴细胞的DNA复制、转录、翻译、糖酵解、柠檬酸循环和丙酮酸代谢等一系列过程中的活性放缓。这项工作揭示了石榴细胞在弱光和低温条件下的分子机制的一些基本信息，为进一步研究大规模绿潮的种子来源提供了有用的线索。
The past several years witnessed the increasing global interest in the marine green macroalga Ulva prolifera as it is a key causative species of the massive green tides successively occurring in the Yellow Sea. Accurate localization of the 'seed' source is one of the principal scientific concerns to be solved before it is possible to manage these algal blooms. It has been suggested that somatic cells of Ulva prolifera which settled in cold benthic sediments might serve as one of the major propagule banks. To identify the molecular mechanisms underlying this hypothesis, PCR-based suppression subtractive hybridization was employed to analyze the differential gene expression of Ulva prolifera under low light and low temperature conditions (matching the cold benthic sediments conditions, 6 A degrees C, 30 mu mol photons m(-2) s(-1)). 137 ESTs representing 88 unigenes (80 singletons and 8 contigs) were detected as being over-expressed, whereas 109 unigenes (96 singletons and 13 contigs) in 130 ESTs were found to be down-regulated in this study. BLASTX analysis revealed that 65 % of the over-expressed and 59 % of the down-regulated genes did not belong to any documented functionally annotated or hypothetical proteins in the public database. However, analysis of the functional defined sequences displayed (1) an obvious sign of senescence, (2) enhancements of the photosynthesis system and the pentose phosphate pathway, (3) slow-down of activities in a wide range of processes including the DNA replication, the transcription, the translation, the glycolysis, the citrate cycle and the pyruvate metabolism in Ulva prolifera cells under low light and low temperature conditions. This work disclosed some basic information of the molecular mechanisms of Ulva prolifera cells under low light and low temperature conditions and provides useful clues for future studies on the "seed" source of the massive green tides.