PKC-SWI6 signaling regulates asexual development, cell wall integrity, stress response, and lifestyle transition in the nematode-trapping fungus Arthrobotrys oligospora
PKC-SWI6 signaling regulates asexual development, cell wall integrity, stress response, and lifestyle transition in the nematode-trapping fungus Arthrobotrys oligospora
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DOI：
10.1007/s11427-022-2118-0
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Meihua Xie;N. Ma;N. Bai;Le Yang;Xuewei Yang;Kecheng Zhang;Jinkui Yang
中科院分区：
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作者：
Meihua Xie;N. Ma;N. Bai;Le Yang;Xuewei Yang;Kecheng Zhang;Jinkui Yang
捕食性真菌具有复杂的信号转导系统，调节其发育并支持宿主的成功感染。在本文中，我们使用基因破坏和多组学方法，在一种代表性的捕食线虫真菌少孢节孢菌中表征了细胞壁完整性控制途径的三个组分，即蛋白激酶C（AoPKC）、SLT 2-MAPK（AoSLT 2）和SWI 6（AoSWI 6）。Δ Aopkc和Δ Aoswi 6突变体的表型性状（如菌丝发育、分生孢子形成、胁迫反应和陷阱形态发生）和代谢特征与Δ Aoslt 2突变体相似，但不同。转录组学分析表明，在Aoswi 6缺失的情况下差异表达的基因参与DNA复制、修复和陷阱形成过程中的重组。酵母双杂交实验表明AoPKC与AoSWI 6相互作用，提示inA.在寡孢酵母中，PKC可以直接调节SWI 6，绕过SLT 2信号级联。总之，我们的研究结果加深了我们对捕食线虫真菌无性发育和生活方式转换的调控机制的理解。
Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the host. Herein, we characterized three components of the cell wall integrity-controlling pathway, namely protein kinase C (AoPKC), SLT2-MAPK (AoSLT2), and SWI6 (AoSWI6), in a representative nematode-trapping fungusArthrobotrys oligospora, using gene disruption and multi-omics approaches. The phenotypic traits (such as mycelia development, conidiation, stress response, and trap morphogenesis) and metabolic profiles of ΔAopkcand ΔAoswi6mutants were similar but differed from those of the ΔAoslt2mutants. Transcriptomic analysis indicated that the genes differentially expressed in the absence ofAoswi6were involved in DNA replication, repair, and recombination during trap formation. Moreover, the yeast two-hybrid assay showed that AoPKC interacted with AoSWI6, suggesting that inA. oligospora, PKC can directly regulate SWI6, bypassing the SLT2 signaling cascade. Conclusively, our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.