Glycodendrimers as new tools in the search for effective anti-HIV DC-based immunotherapies
Glycodendrimers as new tools in the search for effective anti-HIV DC-based immunotherapies
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DOI：
10.1016/j.nano.2013.03.004
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发表时间：
2013-10-01
影响因子：
5.4
通讯作者：
Munoz-Fernandez, M. A.
中科院分区：
文献类型：
--
作者：
Cordoba, E. Vacas;Pion, M.;Munoz-Fernandez, M. A.
树突状细胞 (DC) 在细胞介导的免疫发展中发挥着重要作用，为开发新型抗 HIV 疫苗提供了机会。树枝状聚合物已被提议作为改善 DC 抗原负载的新载体，通过这种方式，我们确定了麦芽糖修饰的中性和带正电荷的 G4 糖树枝状聚合物的潜在用途。因此，在 HIV 感染的背景下评估了这些糖树枝状聚合物对人类 DC 的免疫刺激特性。我们已经证明，用糖树枝状聚合物处理的 DC 在活力、成熟和 HIV 衍生抗原的摄取方面具有完全的功能。然而，iDC 和 mDC 表型以及 mDC 功能（例如迁移能力和细胞因子谱产生）发生了变化。我们的结果显示了糖树枝状聚合物通过直流刺激激活免疫系统的潜在载体特性。这是第一项探索使用麦芽糖功能化树枝状大分子-肽复合物作为潜在的 DC 疫苗候选物的研究。 (C) 2013 Elsevier Inc. 保留所有权利。
Dendritic cells (DC), which play a major role in development of cell-mediated immunity, represent opportunities to develop novel anti-HIV vaccines. Dendrimers have been proposed as new carriers to ameliorate DC antigen loading and in this way, we have determined the potential use of maltose decorated neutrally and positively charged G4 glycodendrimers. Thus, immunostimulatory properties of these glycodendrimers on human DC were evaluated in the context of HIV infection. We have demonstrated that DC treated with glycodendrimers were fully functional with respect to viability, maturation and HIV-derived antigens uptake. Nevertheless, iDC and mDC phenotypes as well as mDC functions such as migration ability and cytokines profile production were changed. Our results showed the potential carrier properties of glycodendrimers to activate the immune system by the way of DC stimulation. This is the first study for exploring the use of maltose-functionalized dendrimers-peptides complexes as a potential DC-based vaccine candidate. (C) 2013 Elsevier Inc. All rights reserved.