Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis.
Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis.
复制标题
丁子香酚通过抑制炎症反应和细胞凋亡来保护大鼠移植心脏免受缺血/再灌注损伤。
DOI：
10.3892/etm.2018.6598
复制
发表时间：
2018-10
影响因子：
2.7
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Feng W;Jin L;Xie Q;Huang L;Jiang Z;Ji Y;Li C;Yang L;Wang D
本研究旨在探讨丁香酚对移植心脏的保护作用及其机制。雄性Sprague-Dawley大鼠随机分为假手术组（n=10）、丁香酚组（n=10对，供体和受体）和对照组（n=10对，供体和受体）。丁香酚组腹腔注射丁香酚（20 mg/kg/天）。假手术组和对照组腹腔注射等体积生理盐水。15天后，对照组和丁香酚组的受体接受腹部异位心脏移植，而假手术组只接受腹部切开术。术后3 h分别取假手术组、丁香酚组和对照组的原位心脏及外周血进行生化、组织病理学、分子生物学和细胞凋亡分析。与对照组相比，丁香酚治疗组心肌丙二醛含量、血清心肌肌钙蛋白I、肌酸激酶MB、肿瘤坏死因子α和白细胞介素6水平明显降低（P<0.05），心肌损伤明显减轻。Western blot分析显示，丁香酚组细胞裂解型多聚腺苷二磷酸核糖聚合酶1（Poly（ADP-ribose）polymerase 1）、BAX和活性型caspase-3蛋白表达较对照组明显降低，而B细胞淋巴瘤2蛋白表达较对照组明显升高（P<0.05）。丁香酚组心肌细胞凋亡率明显低于对照组（P<0.05）。总之，丁香酚治疗显着减少心肌损伤和移植心脏的保护作用。
The aim of the present study was to investigate the protective effect of eugenol on the transplanted heart and explore its mechanisms of action. Male Sprague-Dawley rats were randomly divided into a sham group (n=10), a eugenol group (n=10 pairs, donors and recipients) and a control group (n=10 pairs, donors and recipients). The recipients in the eugenol group received an intraperitoneal injection of eugenol (20 mg/kg/day). The sham group and the control group received equal volumes of physiological saline by intraperitoneal injection. After 15 days the recipients in the control and eugenol groups underwent abdominal heterotopic heart transplantation, while the sham group received only a coeliotomy. The orthotopic hearts in the sham group and the heterotopic hearts in the eugenol and control groups, as well as the peripheral blood samples from all three groups were taken 3 h post operation for biochemical, histopathological, molecular and apoptosis analyses. Compared with the control group, the eugenol treatment significantly reduced the myocardial malondialdehyde content, serum cardiac troponin I, creatine kinase-MB, tumor necresis factor-α and interleukin-6 levels (P<0.05) and significantly alleviated myocardial injury. Western blot analysis demonstrated that the protein expression of cleaved Poly (ADP-ribose) polymerase 1, BAX and active caspase-3 in the eugenol group were significantly decreased, while B-cell lymphoma 2 expression was significantly increased compared with the control group (P<0.05). The myocardial apoptosis rate of the eugenol group was significantly decreased compared with the control group (P<0.05). In conclusion eugenol treatment significantly reduced myocardial injury and demonstrated protective effects for the transplanted heart.
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