RAPID KINETIC STUDIES OF ACTIVE CA2+ TRANSPORT IN SARCOPLASMIC-RETICULUM
RAPID KINETIC STUDIES OF ACTIVE CA2+ TRANSPORT IN SARCOPLASMIC-RETICULUM
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DOI：
10.1007/bf01869478
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发表时间：
1980-01-01
影响因子：
2.4
通讯作者：
HAYNES, DH
中科院分区：
文献类型：
--
作者：
CHIU, VCK;HAYNES, DH
报道了一种快速、连续监测骨骼肌浆网(SR)中活性钙转运事件的方法。该方法是基于对1-苯胺基-8-萘磺酸盐(ANS-)由于主动转运而导致的荧光增加的定量评估。该方法的时间分辨率为20ms，应用于富含Ca~(2+)-ATPase的SR组分的Ca~(2+)转运动力学研究，考察了Mg~(2+)和单价阳离子(M~+)的作用及其作用部位。在没有沉淀阴离子络合作用的情况下，考察了酶的周转数及其建立梯度的能力。这些结果可以用金泽等人的模型来解释。并且很难与酶被认为是产电的模型相一致。
A method is reported for the rapid and continuous monitoring of active Ca2+ transport events occurring in isolated skeletal sarcoplasmic reticulum (SR). The method is based on the quantitative evaluation of increases in the fluorescence of 1-anilino-8-naphthalenesulfonate (ANS-), resulting from active transport. The method, which has a time resolution of 20 ms, was applied to the kinetics of Ca2+ transport by a Ca2+-ATPase-rich SR fraction and the effects and loci of action of Mg2+ and monovalent cations (M+) were investigated. The turnover number of the enzyme and its ability to establish gradients were investigated in the absence of the complicating effects of precipitating anions. The results are explicable in terms of the model of Kanazawa, et al. and are difficult to reconcile with models in which the enzyme is considered to be electrogenic.