Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains.
Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains.
复制标题
从天然组织中纯化目标蛋白：来自牛睾丸的 CCT 复合物和来自猪脑的 PP2Ac。
DOI：
10.1007/7651_2017_89
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Xing,Yongna
中科院分区：
文献类型：
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作者：
Guo,Feng;Wlodarchak,Nathan;Menden,Patrick;Xing,Yongna
蛋白质组学是一种强有力的方法，用于系统地鉴定和定量生物系统（细胞、组织、器官、生物流体或生物体）在特定时间点（ http://www.nature.com ).从天然组织中提取和纯化目的蛋白是蛋白质组学研究的重要步骤。在本章中，我们将介绍从两种不同组织中获得可溶性蛋白的实验方法：（1）从牛睾丸中获得CCT（含cpn的TCP-1）复合物;（2）从猪脑中获得蛋白磷酸酶2A（PP 2A）催化亚基（PP 2Ac或C）。通过这两个例子，我们希望为研究人员提供一些关于如何从特定组织中提取和纯化靶蛋白的一般指导方针，并将这些方法扩展到其他感兴趣的蛋白质。
Proteomics is a powerful approach for systematic identification and quantification of the entire proteome of a biological system (cell, tissue, organ, biological fluid, or organism) at specific time points ( http://www.nature.com ). Extracting and purifying target proteins from native tissues are essential steps for many aspects of proteomic studies. In this chapter, we will introduce the experimental procedures to obtain soluble proteins from two different tissues: (1) the CCT (cpn-containing TCP-1) complex from bovine testes and (2) the protein phosphatase 2A (PP2A) catalytic subunit (PP2Ac or C) from porcine brains. With these two examples, we would like to provide some general guidelines for researchers on how to extract and purify target proteins from specific tissues and extend these approaches to other proteins of interest.