Integrated DNA and Fingerprint Analyses in the Identification of 60‐Year‐Old Mummified Human Remains Discovered in an Alaskan Glacier
Integrated DNA and Fingerprint Analyses in the Identification of 60‐Year‐Old Mummified Human Remains Discovered in an Alaskan Glacier
复制标题
综合 DNA 和指纹分析鉴定阿拉斯加冰川中发现的 60 岁木乃伊人类遗骸
DOI：
--
复制
发表时间：
2010
影响因子：
1.6
通讯作者：
Edward M. Robinson
中科院分区：
文献类型：
--
作者：
O. Loreille;R. Parr;Kevin A. McGregor;Colleen Fitzpatrick;Chriss Lyon;Dongya Y. Yang;C. Speller;Michael R. Grimm;Michael J. Grimm;J. Irwin;Edward M. Robinson
翻译后摘要：这份报告描述了一个商船水手谁在1948年死亡时，西北航空公司4422号航班，一个DC-4携带24名海员和6名机组人员坠毁在桑福德山，阿拉斯加州。 51年后，在飞机残骸附近发现了一个人的前臂和手，促使人们使用DNA和指纹进行鉴定。指纹和DNA分析都面临重大挑战。这只手严重干燥，使得指纹摩擦脊细节几乎看不见，遗骸在发现时已经防腐，使得DNA扩增困难。我们提出了一个跨学科的方法，成功地解决了这些挑战，并最终导致遗体的识别结果。这些努力依赖于有效的指纹复原和成像技术，提高了指纹分辨率，以及针对积极防腐遗体优化的新DNA提取技术。
Abstract: This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC‐4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty‐one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identification efforts using DNA and fingerprints. There were significant challenges to both the fingerprint and DNA analyses. The hand was badly desiccated, making fingerprint friction‐ridge detail almost invisible and the remains had been embalmed upon discovery, making DNA amplification difficult. We present the results of an interdisciplinary approach that successfully addressed these challenges and ultimately led to the identification of the remains. These efforts relied on efficient fingerprint rejuvenation and imaging techniques that improved print resolution, as well as new DNA extraction techniques optimized for aggressively embalmed remains.