Automated high-throughput mouse transsynaptic viral tracing using iDISCO+ tissue clearing, light-sheet microscopy, and BrainPipe.
Automated high-throughput mouse transsynaptic viral tracing using iDISCO+ tissue clearing, light-sheet microscopy, and BrainPipe.
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DOI：
10.1016/j.xpro.2022.101289
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Wang, Samuel S-H
中科院分区：
文献类型：
--
作者：
Pisano, Thomas J.;Hoag, Austin T.;Dhanerawala, Zahra M.;Guariglia, Sara R.;Jung, Caroline;Boele, Henk-Jan;Seagraves, Kelly M.;Verpeut, Jessica L.;Wang, Samuel S-H
跨突触病毒追踪需要组织切片、手工细胞计数和解剖分配，所有这些都是耗时的。我们描述了BrainPipe的协议，BrainPipe是一种可扩展的软件，用于自动解剖对齐和轻片显微镜体积中的物体计数。BrainPipe可以通过使用新的图谱和训练用于目标检测的神经网络来推广到新的计数任务。结合病毒追踪、iDISCO+组织清除和BrainPipe，有助于绘制小脑与小鼠大脑其他部分的连接图。有关使用和执行本协议的完整详情，请参阅。结合病毒追踪、iDISCO+和BrainPipe进行高通量的跨突触研究，跨突触病毒追踪需要组织切片、人工细胞计数和解剖分配，所有这些都是耗时的。我们描述了BrainPipe的协议，BrainPipe是一种可扩展的软件，用于自动解剖对齐和轻片显微镜体积中的物体计数。BrainPipe可以通过使用新的图谱和训练用于目标检测的神经网络来推广到新的计数任务。结合病毒追踪、iDISCO+组织清除和BrainPipe，有助于绘制小脑与小鼠大脑其他部分的连接图。
Transsynaptic viral tracing requires tissue sectioning, manual cell counting, and anatomical assignment, all of which are time intensive. We describe a protocol for BrainPipe, a scalable software for automated anatomical alignment and object counting in light-sheet microscopy volumes. BrainPipe can be generalized to new counting tasks by using a new atlas and training a neural network for object detection. Combining viral tracing, iDISCO+ tissue clearing, and BrainPipe facilitates mapping of cerebellar connectivity to the rest of the murine brain. For complete details on the use and execution of this protocol, please refer to. Using iDISCO+ whole-brain tissue clearing technique for transsynaptic viral tracing BrainPipe enables automated registration and analysis of light-sheet microscopy volumes Combining viral tracing, iDISCO+, and BrainPipe for high-throughput transsynaptic study Transsynaptic viral tracing requires tissue sectioning, manual cell counting, and anatomical assignment, all of which are time intensive. We describe a protocol for BrainPipe, a scalable software for automated anatomical alignment and object counting in light-sheet microscopy volumes. BrainPipe can be generalized to new counting tasks by using a new atlas and training a neural network for object detection. Combining viral tracing, iDISCO+ tissue clearing, and BrainPipe facilitates mapping of cerebellar connectivity to the rest of the murine brain.
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影响因子：
12.4
作者：
Mastakov, MY;Baer, K;During, MJ
通讯作者：
During, MJ
DOI：
10.3791/3564
发表时间：
2012-07-30
期刊：
Journal of visualized experiments : JoVE
影响因子：
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作者：
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影响因子：
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作者：
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