Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture
Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture
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DOI：
10.1385/bter:104:1:057
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发表时间：
2005-04-01
影响因子：
3.9
通讯作者：
Minami, T
中科院分区：
文献类型：
--
作者：
Dohi, Y;Shimaoka, H;Minami, T
研究了地塞米松治疗后大鼠骨髓间充质干细胞（MSCs）传代培养中金属硫蛋白（MT）亚型mrna的时间变化。MT-1。地塞米松培养MSCs中MT-2 mRNA的表达在第1天达到最高水平，而ALP和骨钙素mRNA在第12天达到峰值。任何时候培养的MSCs中均未检测到MT-3 mRNA。第1天MT-2 mRNA的表达量是MT-1 mRNA的9.4倍。最后，在第14天，在培养基中添加MT-1和MT-2的共同反义寡核苷酸，可以抑制MSCs的成骨细胞分化和矿化。结果表明，MSCs在传代培养早期产生了大量的MT-2，这可能调节了MSCs的分化。
Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1. and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d I was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation.