An Integrated Badnavirus Is Prevalent in Fig Germplasm
An Integrated Badnavirus Is Prevalent in Fig Germplasm
复制标题
DOI：
10.1094/phyto-12-11-0351
复制
发表时间：
2012-12-01
期刊：
影响因子：
3.2
通讯作者：
Tzanetakis, Ioannis E.
中科院分区：
文献类型：
--
作者：
Laney, Alma G.;Hassan, Mohamed;Tzanetakis, Ioannis E.
无花果花叶病毒病发生在世界各地，是无花果最常见和最重要的病毒性疾病。在寻求确定疾病的病原体，已经确定了几种新的病毒，包括一种新的DNA病毒，这次交流的主题。系统发育分析将该病毒暂时命名为Fig badnavirus-1（FBV-1），属于花椰菜病毒科Badnavirus属。评价了该病毒的实验宿主范围，并将其机械传播到几种草本宿主。在国家克隆种质库无花果收集和来自阿肯色州、加州、佛罗里达、密歇根州、俄亥俄州、俄勒冈州和南卡罗来纳州的额外样品中检测到FBV-1，表明其在美国广泛分布。进一步的测试揭示了在幼苗和分生组织培养植物中存在FBV-1。在一项评估美国病毒种群结构的研究中使用了44个分离株。证据表明，FBV-1是集成在无花果基因组中提出和讨论。
Fig mosaic occurs worldwide and is the most common and important viral disease of fig. In the quest to identify the causal agent of the disease, several new viruses have been identified, including a new DNA virus, the subject of this communication. Phylogenetic analysis placed the virus, provisionally named Fig badnavirus-1 (FBV-1), in the genus Badnavirus, family Caulimoviridae. The experimental host range of FBV-1 was evaluated and the virus was mechanically transmitted to several herbaceous hosts. FBV-1 was detected in the National Clonal Germplasm Repository Fig collection and additional samples from Arkansas, California, Florida, Michigan, Ohio, Oregon, and South Carolina, suggesting its wide distribution in the United States. Further tests revealed the presence of FBV-1 in seedlings and meristem tissue culture plants. Forty-four isolates were used in a study evaluating the population structure of the virus in the United States. Evidence that FBV-1 is integrated in the fig genome is presented and discussed.