Interferometric Gated Off-Axis Reflectometry (iGOR) - A Label Free Method to Measure Lipid Membrane Dynamics and Deduce Biophysical Properties
Interferometric Gated Off-Axis Reflectometry (iGOR) - A Label Free Method to Measure Lipid Membrane Dynamics and Deduce Biophysical Properties
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干涉门控离轴反射计 (iGOR) - 一种测量脂质膜动力学并推断生物物理特性的无标记方法
DOI：
10.1109/cleo/europe-eqec57999.2023.10232035
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Turley F
中科院分区：
文献类型：
--
作者：
Turley F
细胞膜内蛋白质的功能和组织是细胞生物学中的一个基本领域，支持关键过程。当研究蛋白质和脂质膜之间的相互作用时，重要的是要考虑动态系统，因为已知蛋白质和膜相行为可以调节彼此的功能[1]。然而，由于技术上的限制，膜动力学的研究往往是福尔斯短，特别是在评估厚度和曲率。传统的荧光方法存在光漂白和光毒性[2]。iSCAT和质量光度法等无标记技术允许在支持的脂质双层上观察膜组分，这不可避免地抑制了膜的波动[3，4]。我们已经开发了一种技术，干涉门控离轴反射（iGOR），以减轻这些限制，方便精确观察的一部分悬浮的脂质膜。iGOR是数字全息术一种形式（见图1a，c），其中我们将膜厚度和高度的联合收割机测量结合起来，利用热波动分析膜刚度和相变。为了最大限度地提高时间分辨率，我们的系统在327 Hz下采集，曝光时间为3 ms。
The function and organisation of proteins within the cell membrane is a fundamental area within cell biology, underpinning key processes. When investigating the interaction between proteins and lipid membranes, it is important to consider a dynamic system, as proteins and membrane phase behaviour are known to modulate the function of each other [1]. However, due to limits in technology, the study of membrane dynamics often falls short, especially when evaluating thickness and curvature. Traditional fluorescence methods suffer from photobleaching and phototoxicity [2]. Label-free techniques like iSCAT and mass photometry allow the observation of membrane components on a supported lipid bilayer which inevitably dampens fluctuations of the membrane [3, 4]. We have developed a technique, Interferometric gated off-axis reflectometry (iGOR), to alleviate these limitations by facilitating precise observation of a section of suspended lipid membrane. iGOR is a form of digital holography (see Fig. 1a, c) in which we combine measurements of membrane thickness and height to analyse membrane stiffness and phase transitions using thermal fluctuations. To maximise the temporal resolution our system acquires at 327Hz with an exposure time of 3ms.
影响因子：
7.3
作者：
P. Sengupta;B. Baird;D. Holowka
通讯作者：
P. Sengupta;B. Baird;D. Holowka