Hitchhiking vesicular transport routes to the vacuole: Amyloid recruitment to the Insoluble Protein Deposit (IPOD)
Hitchhiking vesicular transport routes to the vacuole: Amyloid recruitment to the Insoluble Protein Deposit (IPOD)
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搭便车到达液泡的囊泡运输路线：淀粉样蛋白募集到不溶性蛋白沉积物（IPOD）
DOI：
10.1080/19336896.2017.1293226
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发表时间：
2017
期刊：
影响因子：
2.3
通讯作者：
Tyedmers J
中科院分区：
文献类型：
--
作者：
Kumar R;Neuser N;Tyedmers J
聚集体螯合到专门的沉积位点发生在从细菌到哺乳动物的所有生命界的许多物种中，并且通常被认为具有细胞保护功能。酵母细胞具有至少3个不同的空间上分离的沉积位点，其中之一被称为"不溶性蛋白质存款（IPOD）"并含有淀粉样蛋白聚集体。我们最近发现，淀粉样蛋白聚集体的招募IPOD使用肌动蛋白电缆为基础的招聘机制，也涉及囊泡运输。在这里，我们讨论了不同的蛋白质参与囊泡运输过程中的液泡可能会采取行动，引导淀粉样蛋白聚集的IPOD。这些因子包括参与沿沿着肌动蛋白缆运输液泡囊泡的肌球蛋白V马达蛋白Myo2、参与将大的前体水解酶复合物募集到液泡的跨膜蛋白Atg 9、磷脂酰肌醇/磷脂酰胆碱（PI/PC）转移蛋白Sec 14和SNARE伴侣蛋白Sec 18。此外，我们提出了新的数据表明，酵母动力蛋白同源物Vps1也是至关重要的忠实交付的淀粉样蛋白模型蛋白PrD-GFP的IPOD。这与先前确定的Vps1在招募热变性聚集体到液泡周围沉积位点中的作用一致。
Sequestration of aggregates into specialized deposition sites occurs in many species across all kingdoms of life ranging from bacteria to mammals and is commonly believed to have a cytoprotective function. Yeast cells possess at least 3 different spatially separated deposition sites, one of which is termed “Insoluble Protein Deposit (IPOD)” and harbors amyloid aggregates. We have recently discovered that recruitment of amyloid aggregates to the IPOD uses an actin cable based recruitment machinery that also involves vesicular transport. Here we discuss how different proteins known to be involved in vesicular transport processes to the vacuole might act to guide amyloid aggregates to the IPOD. These factors include the Myosin V motor protein Myo2 involved in transporting vacuolar vesicles along actin cables, the transmembrane protein Atg9 involved in the recruitment of large precursor hydrolase complexes to the vacuole, the phosphatidylinositol/ phosphatidylcholine (PI/PC) transfer protein Sec 14 and the SNARE chaperone Sec 18. Furthermore, we present new data suggesting that the yeast dynamin homolog Vps1 is also crucial for faithful delivery of the amyloid model protein PrD-GFP to the IPOD. This is in agreement with a previously identified role for Vps1 in recruitment of heat-denatured aggregates to a perivacuolar deposition site.
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影响因子：
3.3
作者：
Reggiori, F;Monastyrska, L;Klionsky, DJ
通讯作者：
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