EGFR-Targeted Magnetic Nanoparticle Heaters Kill Cancer Cells without a Perceptible Temperature Rise
EGFR-Targeted Magnetic Nanoparticle Heaters Kill Cancer Cells without a Perceptible Temperature Rise
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DOI：
10.1021/nn201822b
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发表时间：
2011-09-01
期刊：
影响因子：
17.1
通讯作者：
Rinaldi, Carlos
中科院分区：
文献类型：
--
作者：
Creixell, Mar;Bohorquez, Ana C.;Rinaldi, Carlos
目前认为，磁性纳米颗粒加热器（MNH）只有在温度升高到43摄氏度以上时才能杀死癌细胞，这是由于交变磁场中的能量耗散。另一方面，简单的热传导参数表明，在小肿瘤或单细胞中，能量耗散和热传导的相对速率导致可忽略的温度升高，从而限制了治疗小肿瘤和转移性癌症的潜在eMNH。在这里，我们证明，内化的MNH共轭表皮生长因子（EGF）和靶向表皮生长因子受体（EGFR）的结果在一个显着的（高达99.9%）减少细胞活力和克隆存活率在一个热剂量依赖性的方式，而不需要一个可感知的温度上升。这种效应似乎是细胞类型特异性的，表明交变磁场中的磁性纳米颗粒可以在以前认为不可能的条件下有效地杀死癌细胞。
It is currently believed that magnetic nanoparticle heaters (MNHs) can kill cancer cells only when the temperature is raised above 43 degrees C due to energy dissipation in an alternating magnetic field. On the other hand, simple heat conduction arguments indicate that in small tumors or single cells the relative rates of energy dissipation and heat conduction result in a negligible temperature rise, thus limiting the potential e MNHs in treating small tumors and metastatic cancer. Here we demonstrate that internalized MNHs conjugated to epidermal growth factor (EGF) and which target the epidermal growth factor receptor (EGFR) do result in a significant (up to 99.9%) reduction in cell viability and clonogenic survival in a thermal heat dose dependent manner, without the need for a perceptible temperature rise. The effect appears to be cell type specific and indicates that magnetic nanoparticles in alternating magnetic fields may effectively kill cancer cells under conditions previously considered as not possible.