Modification of Daxx by small ubiquitin-related modifier-1
Modification of Daxx by small ubiquitin-related modifier-1
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DOI：
10.1016/s0006-291x(02)00699-x
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发表时间：
2002-07-12
影响因子：
3.1
通讯作者：
Kim, E
中科院分区：
文献类型：
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作者：
Jang, MS;Ryu, SW;Kim, E
小泛素相关修饰物-1 (SUMO-1) 是一种与多种细胞蛋白共价修饰的蛋白质，可保护细胞免受抗 Fas 和 TNF 诱导的细胞死亡。之前，我们报道了 Daxx 的 C 末端与 Ubc9（一种 E2 型 SUMO-I 缀合酶）以及 SUMO-1 相互作用。在表达 FLAG-Daxx 和 HA-SUMO-1 的 BOSC23 细胞中，出现 110 和 130 kDa Daxx，并且 130kDa 带与抗 HA 和抗 FLAG 抗体结合。这意味着Daxx可以被SUMO-L共价修饰，K630和K631的取代消除了SUMO1对Daxx的修饰，这意味着K630和K631对于SUMO化是必需的。 Daxx (K630, 631A) 和 Daxx (K634, 636, 637A) 的假定 C 端核定位信号 (NLS) 被破坏，出现在细胞核中，表明 C 端 NLS 没有功能。 Daxx (K630, 631A) 是 sumoylation 缺陷突变体，能够与 PML 相互作用并与 PML 共定位于 PML 致癌结构域 (POD)。因此，我们的数据表明 Daxx 的苏酰化状态不会影响其在 POD 中的存在。 (C) 2002 年爱思唯尔科学（美国）。版权所有。
Small ubiquitin-related modifier-1 (SUMO-1) is a protein that is covalently modified to various cellular proteins and protects cells against both anti-Fas and TNF-induced cell death. Previously, we reported that the C-terminus of Daxx interacted with Ubc9, an E2 type SUMO-I conjugating enzyme, as well as with SUMO-1. In BOSC23 cells expressing FLAG-Daxx together with HA-SUMO-1, 110 and 130 kDa Daxx appeared and the 130kDa band bound to both anti-HA and anti-FLAG antibodies. This means that Daxx can be covalently modified by SUMO-L Substitution of K630 and K631 abrogated the modification of Daxx by SUMO1, implying that K630 and K631 were essential for sumoylation. Daxx (K630, 631A) and Daxx (K634, 636, 637A) in which the putative C-terminal nuclear localization signals (NLSs) were disrupted appeared in the nucleus, suggesting that the C-terminal NLS was not functional. Daxx (K630, 631A), the sumoylation defective mutant, was able to interact with PML and co-localized with PML in the PML oncogenic domains (PODs). Thus, our data show that sumoylation status of Daxx does not affect its presence in PODs. (C) 2002 Elsevier Science (USA). All rights reserved.