Grain-rich diets altered the colonic fermentation and mucosa-associated bacterial communities and induced mucosal injuries in goats.
Grain-rich diets altered the colonic fermentation and mucosa-associated bacterial communities and induced mucosal injuries in goats.
复制标题
富含谷物的饮食改变了山羊的结肠发酵和粘膜相关细菌群落，并引起粘膜损伤
DOI：
10.1038/srep20329
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发表时间：
2016-02-04
影响因子：
4.6
通讯作者：
Mao S
中科院分区：
文献类型：
--
作者：
Ye H;Liu J;Feng P;Zhu W;Mao S
关于高谷物（HG）喂养对结肠粘膜相关细菌和粘膜形态学的影响的信息非常少。在本研究中，12只雄性山羊被随机分配到干草饮食（n = 6）或HG饮食（65%谷物; n = 6），以观察结肠粘膜细菌群落组成和结肠粘膜形态的变化。结果表明，饲喂HG降低了结肠pH值，增加了结肠粘膜中总短链脂肪酸和脂多糖的含量。主坐标分析结果表明，HG饮食改变了结肠粘膜细菌群落，布劳特氏菌属的丰度增加，芽孢杆菌属、肠球菌属和乳球菌属的丰度减少。结果表明，饲喂HG的山羊结肠粘膜上皮细胞表面脱落，细胞间紧密连接破坏，细胞线粒体损伤，IL-2和IFN-γ相对mRNA表达上调。总的来说，我们的数据表明，HG喂养诱导结肠粘膜形态和细胞因子表达的变化，这可能是由过度发酵和结肠中细菌种群的急剧变化引起的。
Remarkably little information is available about the impact of high-grain (HG) feeding on colonic mucosa-associated bacteria and mucosal morphology. In the present study, 12 male goats were randomly assigned to either a hay diet (n = 6) or an HG diet (65% grain; n = 6) to characterise the changes in the composition of the bacterial community in colonic mucosa and the mucosal morphology of the colon. The results showed that HG feeding decreased the colonic pH and increased the concentrations of total short chain fatty acids and lipopolysaccharides in colonic digesta. The principal coordinate analysis results showed that the HG diet altered the colonic mucosal bacterial communities, with an increase in the abundance of genusBlautiaand a decrease in the abundance of generaBacillus, Enterococcus,andLactococcus. The HG-fed goats showed sloughing of the surface layer epithelium, intercellular tight junction erosion, cell mitochondrial damage and upregulation of the relative mRNA expression of IL-2 and IFN-γ in colonic mucosa. Collectively, our data indicate that HG feeding induced changes in colonic mucosal morphology and cytokines expression that might be caused by excessive fermentation and dramatic shifts in the bacterial populations in the colon.