Alterations in osteoclast morphology following osteoprotegerin administration in the magnesium-deficient mouse
Alterations in osteoclast morphology following osteoprotegerin administration in the magnesium-deficient mouse
复制标题
缺镁小鼠给予骨保护素后破骨细胞形态的变化
DOI：
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发表时间：
2003
影响因子：
1.6
通讯作者：
RK Rude
中科院分区：
文献类型：
--
作者：
H. Gruber;RK Rude
在本研究中，我们使用骨保护素（OPG），它阻止破骨细胞的形成，以纠正，从而解释高钙血症，这是在膳食镁缺乏的小鼠。对照组和Mg缺乏组小鼠仅接受OPG或溶媒注射12天。接受OPG处理的Mg缺乏小鼠和接受OPG的对照小鼠的血清Ca相似（9.2±0.3 mg/dl vs. 9.2±0.5）。两组的血清Ca均显著高于对照组或仅接受溶媒的Mg缺乏动物。令人惊讶的是，接受OPG抑制骨吸收剂量的Mg耗尽小鼠仍然高钙。由于骨髓中存在的成熟破骨细胞可能是过度活跃的，我们在光镜和超微结构水平上检查了破骨细胞的形态。骨小梁的光学显微镜检查显示OPG处理的小鼠中几乎没有破骨细胞。超微结构检查显示，OPG处理的小鼠中的破骨细胞与骨内膜骨表面的接触减少，并且没有皱褶边缘。由于现有的成熟破骨细胞池的形态并没有增强再吸收，另一种机制，如镁缺乏小鼠中钙的肠吸收增加，可能有助于在镁缺乏期间观察到的高钙血症。
In the present study, we used osteoprotegerin (OPG), which blocks osteoclastogenesis, to correct and thus explain the hypercalcemia that is seen during dietary Mg deficiency in the mouse. Control and Mg-deficient mice received injections for 12 days of either OPG or vehicle only. Serum Ca was similar in Mg-deficient mice treated with OPG and in control mice receiving OPG (9.2±0.3 mg/dl vs. 9.2±0.5). Both groups had significantly higher serum Ca than controls or Mg-deficient animals receiving vehicle alone. Surprisingly, Mg-depleted mice that received OPG in doses that inhibit osteoclastic bone resorption remained hypercalcemic. Because mature osteoclasts still present in the marrow might be hyperactive, we examined osteoclast morphology at the light microscopic and ultrastructural level. Light microscopic examination of trabecular bone showed few osteoclasts in OPG-treated mice. Ultrastructural examination revealed that osteoclasts in OPG-treated mice have decreased contact with the endosteal bone surface and absence of a ruffled border. Because the morphology of the existing pool of mature osteoclasts did not enhance resorption, another mechanism, such as increased intestinal absorption of Ca in Mg-deficient mice, likely contributes to the hypercalcemia observed during Mg deficiency.
生活在夏威夷的老年日裔美国男性和女性的饮食与多个骨骼部位的骨矿物质含量之间的关系。
DOI：
10.1093/ajcn/42.5.877
发表时间：
1985
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Yano,K;Heilbrun,LK;Wasnich,RD;Hankin,JH;Vogel,JM
通讯作者：
Vogel,JM
影响因子：
4.8
作者：
Gori, F;Hofbauer, LC;Riggs, BL
通讯作者：
Riggs, BL