Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion.
Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion.
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DOI：
10.1186/s13550-015-0152-9
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发表时间：
2015-12
期刊：
影响因子：
3.2
通讯作者：
Kinuya S
中科院分区：
文献类型：
--
作者：
Wakabayashi H;Taki J;Inaki A;Shiba K;Matsunari I;Kinuya S
为了研究心肌梗死后99 mTc-annexin-V（99 mTc-AV）摄取所显示的凋亡过程是否与左心室重构（LVR）相关，我们评估了大鼠心肌缺血和再灌注模型的99 mTc-AV摄取。结扎15只大鼠左冠状动脉20 ~ 30 min后再灌注。2周后注射99 mTc-AV，1h后再阻断左冠状动脉后注射201 Tl。双示踪剂放射自显影评估99 mTc-AV摄取和201 Tl缺损的危险区域（AAR）。99 mTc-AV摄取率的计算方法为AAR的计数密度除以正常灌注面积的计数密度。在短轴左心室切片中，通过将左心室腔面积除以整个左心室面积来计算左心室腔扩张指数（DI）。左室壁薄化率（WTR）的计算方法是将AAR中的左室壁厚度除以正常灌注面积。在10只大鼠的AAR中观察到显著的99 mTc-AV摄取。99 mTc-AV摄取阳性大鼠的DI显著高于未摄取的大鼠。99 mTc-AV摄取阳性大鼠的WTR小于未摄取的大鼠。结果提示，心肌缺血再灌注后2周，损伤心肌的99 mTc-AV摄取与LVR有关。
To investigate whether an apoptotic process demonstrated by 99mTc-annexin-V (99mTc-AV) uptake correlates with left ventricular remodeling (LVR) after myocardial infarction, we assessed 99mTc-AV uptake in rat model of myocardial ischemia and reperfusion. The left coronary artery (LCA) of 15 rats was occluded for 20 to 30 min, followed by reperfusion. After 2 weeks, 99mTc-AV was injected, and then 1 h later, 201Tl was injected after reocclusion of the LCA. Dual-tracer autoradiography was performed to assess 99mTc-AV uptake and the area at risk (AAR) by 201Tl defect. 99mTc-AV uptake ratio was calculated by dividing the count density of the AAR by that of the normally perfused area. In short-axis LV slices, LV cavity dilation index (DI) was calculated by dividing the area of LV cavity by that of the whole LV area. LV wall-thinning ratio (WTR) was calculated by dividing the LV wall thickness in the AAR by that of the normally perfused area. Significant 99mTc-AV uptake in the AAR was observed in 10 rats. DI was significantly higher in rats with positive 99mTc-AV uptake than in rats without uptake. WTR was smaller in rats with positive 99mTc-AV uptake than in rats without uptake. The data suggest 99mTc-AV uptake in injured myocardium might correlate with LVR at 2 weeks after myocardial ischemia and reperfusion.