LECTIN-BINDING IN BONE-MATRIX OF ADULT-RATS WITH SPECIAL REFERENCE TO CEMENT LINES
LECTIN-BINDING IN BONE-MATRIX OF ADULT-RATS WITH SPECIAL REFERENCE TO CEMENT LINES
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DOI：
10.1620/tjem.170.81
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发表时间：
1993-06-01
影响因子：
2.2
通讯作者：
AKITA, H
中科院分区：
文献类型：
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作者：
KAGAYAMA, M;SASANO, Y;AKITA, H
本研究的目的是本地化的糖结合物识别的四种凝集素（LFA，MPA，WGA，和刀豆蛋白A）在成年大鼠骨，组织化学和生物化学。LFA是一种识别唾液酸的凝集素，对颅骨骨水泥线和髁突软骨-骨界面有较强的染色，WGA也对这些骨水泥线和髁突软骨-骨界面进行了染色，ConA也对这些骨水泥线和髁突软骨-骨界面进行了间歇性染色。颅骨不规则骨表面LFA和WGA呈强阳性反应，下颌骨髁状突类骨质和颅骨光滑骨表面WGA和ConA呈强阳性反应，LFA和MPA呈弱阳性反应。MPA识别破骨细胞。髁突浅层骨基质LFA呈颗粒状阳性，MPA、WGA、ConA呈片状阳性。骨提取物的凝集素印迹分析显示两个LFA阳性条带，分子量为18 kDa和45 kDa。前者WGA、ConA、MPA阳性，后者MPA阳性。结果表明，LFA结合糖缀合物，可能是18 kDa和/或45 kDa的唾液酸糖缀合物，可能有助于骨水泥线的胶结机制和/或骨重建过程中的骨形成，以及在膜内和软骨内成骨。
The present study was designed to localize glycoconjugates recognized by four lectins (LFA, MPA, WGA, and ConA) in adult rat bone, histochemically and biochemically. LFA, a lectin recognizing sialic acid, stained intensely the cement lines of calvariae and the interface between cartilage and bone of mandibular condyles, which were also stained with WGA, and intermittently stained with ConA. The irregular bone surface of calvariae displayed intense positive reaction with LFA and WGA, while the osteoid of mandibular condyles and the smooth bone surface of calvariae were intensely stained with WGA and ConA, but weakly stained with LFA and MPA. MPA recongnized osteoclasts. At the superficial layer of mandibular condyles, the bone matrix appeared positive as granules with LFA and as patches with MPA, WGA and ConA. Lectin blotting analysis of bone extracts revealed two LFA positive bands at molecular weights of 18 kDa and 45 kDa. The former was positive with WGA, ConA, and MPA, while the latter mas positive with MPA. The result suggests that the LFA-binding glycoconjugates, possibly 18 kDa and/or 45 kDa sialoglycoconjugates, may contribute to cementing mechanism of cement lines and/or to the bone formation in remodeling process as well as in intramembranous and intracartilaginous osteogenesis.