Rotational dynamics of cargos at pauses during axonal transport
Rotational dynamics of cargos at pauses during axonal transport
复制标题
DOI：
10.1038/ncomms2037
复制
发表时间：
2012-08-01
影响因子：
16.6
通讯作者：
Fang, Ning
中科院分区：
文献类型：
--
作者：
Gu, Yan;Sun, Wei;Fang, Ning
活体神经元中轴突运输的直接可视化对于我们理解神经元功能和基于微管的运动蛋白的工作机制是必不可少的。在这里，我们使用高速单粒子取向和旋转跟踪技术，直接可视化货物在主动定向运输和轴突运输的暂停阶段的旋转动力学，时间分辨率为2 ms。长和短的暂停都被成像，以及暂停持续时间，货物在暂停时的旋转行为，以及暂停之后的移动方向。此外，旋转动力学导致切换轨道的详细可视化。这些对货物旋转动力学的首次观察为驱动蛋白和动力蛋白马达如何使货物经历主动定向运输和暂停的交替阶段提供了新的见解。
Direct visualization of axonal transport in live neurons is essential for our understanding of the neuronal functions and the working mechanisms of microtubule-based motor proteins. Here we use the high-speed single particle orientation and rotational tracking technique to directly visualize the rotational dynamics of cargos in both active directional transport and pausing stages of axonal transport, with a temporal resolution of 2 ms. Both long and short pauses are imaged, and the correlations between the pause duration, the rotational behaviour of the cargo at the pause, and the moving direction after the pause are established. Furthermore, the rotational dynamics leading to switching tracks are visualized in detail. These first-time observations of cargo's rotational dynamics provide new insights on how kinesin and dynein motors take the cargo through the alternating stages of active directional transport and pause.