Mitochondria transfer from early stages of erythroblasts to their macrophage niche via tunnelling nanotubes
Mitochondria transfer from early stages of erythroblasts to their macrophage niche via tunnelling nanotubes
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DOI：
10.1111/bjh.17531
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发表时间：
2021-05
影响因子：
6.5
通讯作者：
Chong Yang;Mitsuhiro Endoh;D. Tan;A. Nakamura-Ishizu;Yuji Takihara;T. Matsumura;T. Suda
中科院分区：
文献类型：
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作者：
Chong Yang;Mitsuhiro Endoh;D. Tan;A. Nakamura-Ishizu;Yuji Takihara;T. Matsumura;T. Suda
成人红细胞生成需要一系列良好协调的事件，产生成熟的红细胞。这些事件之一是线粒体清除，其通过自噬依赖机制自主发生。有趣的是，最近的研究显示了线粒体在各种细胞类型之间的转移活动。在红细胞生成的背景下，已知巨噬细胞与成红细胞的早期阶段密切相互作用，以提供称为成红细胞岛（EBI）的特化生态位。然而，线粒体转移是否可以发生在EBI生态位尚未探讨。在这里，我们报告说，线粒体转移EBI利基发生在体内。我们观察了线粒体转移活动的早期阶段的红细胞巨噬细胞在重建的体外小鼠EBI通过不同的模式，包括隧道纳米管（TNT）。此外，我们证明了巨噬细胞中的Wiskott-Aldrich综合征蛋白（WASp）通过调节F-肌动蛋白抑制来介导TNT形成和线粒体转移，从而促进线粒体从红系细胞中清除，以潜在地增强其分化。总之，我们的研究结果提供了新的见解线粒体清除机制，介导红细胞成熟。
Adult erythropoiesis entails a series of well‐coordinated events that produce mature red blood cells. One of such events is the mitochondria clearance that occurs cell‐autonomously via autophagy‐dependent mechanisms. Interestingly, recent studies have shown mitochondria transfer activities between various cell types. In the context of erythropoiesis, macrophages are known to interact closely with the early stages of erythroblasts to provide a specialized niche, termed erythroblastic islands (EBI). However, whether mitochondria transfer can occur in the EBI niche has not been explored. Here, we report that mitochondria transfer in the EBI niche occurs in vivo. We observed mitochondria transfer activities from the early stages of erythroblasts to macrophages in the reconstituted in vitro murine EBI via different modes, including tunnelling nanotubes (TNT). Moreover, we demonstrated that Wiskott‐Aldrich syndrome protein (WASp) in macrophages mediates TNT formation and mitochondria transfer via the modulation of F‐actin filamentation, thus promoting mitochondria clearance from erythroid cells, to potentially enhance their differentiation. Taken together, our findings provide novel insight into the mitochondria clearance machineries that mediate erythroid maturation.