Detecting DNA Loops Using Tethered Particle Motion.
Detecting DNA Loops Using Tethered Particle Motion.
复制标题
使用系留粒子运动检测 DNA 环。
DOI：
10.1007/978-1-0716-3377-9_21
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Dunlap,David
中科院分区：
文献类型：
--
作者：
Qian,Jin;Collette,Dylan;Finzi,Laura;Dunlap,David
由单个聚合物拴系的微米尺寸的珠的运动范围提供了聚合物的有效长度的动态读数。珠的偏移可以反映聚合物的固有柔性和/或拓扑结构以及由于结合聚合物的配体的作用活性而引起的变化。这是一个简单而强大的实验方法来研究DNA和蛋白质之间的相互作用，如用lacrepressor的实验所证明的那样。这种蛋白质形成具有两个结合位点的稳定的四聚体寡聚体，并且可以在沿着DNA分子的长度沿着分开的识别位点之间产生DNA环。
The range of motion of a micron-sized bead tethered by a single polymer provides a dynamic readout of the effective length of the polymer. The excursions of the bead may reflect the intrinsic flexibility and/or topology of the polymer as well as changes due to the action activity of ligands that bind the polymer. This is a simple yet powerful experimental approach to investigate such interactions between DNA and proteins as demonstrated by experiments with thelacrepressor. This protein forms a stable, tetrameric oligomer with two binding sites and can produce a loop of DNA between recognition sites separated along the length of a DNA molecule.