Microtia chondrocytes as a donor source for tissue-engineered cartilage
Microtia chondrocytes as a donor source for tissue-engineered cartilage
复制标题
DOI：
10.1097/01.mlg.0000149455.68135.de
复制
发表时间：
2004-12-01
期刊：
影响因子：
2.6
通讯作者：
Eavey, RD
中科院分区：
文献类型：
--
作者：
Kamil, SH;Vacanti, MP;Eavey, RD
目标/假设。目前矫正小耳畸形的手术技术是具有挑战性的。组织工程学领域的研究正在为未来可能治疗小耳畸形的软骨细胞行为提供洞察力。作者希望评估从小耳软骨分离的软骨细胞作为组织工程软骨来源的生物学潜力，并与正常耳软骨进行比较。研究设计：研究小耳软骨细胞与正常耳软骨细胞作为组织工程软骨来源的潜力的对比研究设计。方法：研究方法。取12例儿童耳软骨标本，其中正常耳标本6例，小耳畸形标本6例。体外分离培养软骨细胞，传代后软骨细胞数量增加。每种类型的细胞都被植入裸鼠体内，以生成组织工程软骨。植入8周后，解剖取出标本。比较了正常耳廓和小耳标本在体外细胞数扩增和体内组织工程软骨生成方面的结果。结果。在第二代结束时，每组(正常和小耳)的平均细胞数从最初的150,000个增加到正常组的平均细胞数1.2亿个/毫升和小耳亚组的1.3亿个细胞/毫升。组织学上，两种类型的软骨细胞都产生了正常的弹性软骨。结论：从小耳软骨中分离出的细胞具有生成组织工程软骨的潜能。小耳软骨是人类组织工程耳廓可能产生的重要额外供体来源。
Objectives/Hypothesis. Current surgical techniques for the correction of microtia are challenging. Research in the field of tissue engineering is providing insight into chondrocyte behavior for a possible future treatment of microtia. The authors wished to evaluate the biological potential of chondrocytes isolated from microtia cartilage as compared with normal auricular cartilage as a source of tissue-engineered cartilage. Study Design: A comparative research design to study the potential of microtia cartilage chondrocytes with normal auricular chondrocytes as a source of tissue-engineered cartilage. Methods. Cartilage specimens from 12 pediatric patients (six normal auricular specimens and six auricular specimens with microtia) were obtained. The chondrocytes were isolated and cultured in vitro; chondrocyte number was increased by passaging. Each type of cell was implanted in nude mice to generate tissue-engineered cartilage. Eight weeks after implantation the specimens were dissected and removed. Results were compared between the normal auricular and microtia specimens in regard to cell number expansion in vitro and generation of tissue-engineered cartilage in vivo. Results. An initial mean cell number of 150,000 cells in each group (normal and microtia) increased to an average cell number of 120 million cells/mL in the normal and 130 million cells in the microtia subgroups, respectively, at the end of the second passage. histologically, both types of chondrocytes generated normal elastic cartilage. Conclusion: The study demonstrated the potential of cells isolated from microtia cartilage to generate tissue-engineered cartilage. Microtia cartilage represents an important additional donor source for the possible generation of a human tissue-engineered auricle.