Anti-cyclic citrullinated peptide antibodies in patients with juvenile idiopathic arthritis
Anti-cyclic citrullinated peptide antibodies in patients with juvenile idiopathic arthritis
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DOI：
10.1080/0891693021000014970
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发表时间：
2002-09-01
期刊：
影响因子：
3.5
通讯作者：
Vavrinec, J
中科院分区：
文献类型：
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作者：
Hromadnikova, I;Stechova, K;Vavrinec, J
我们分析了存在的抗环瓜氨酸肽（抗CCP）和抗角蛋白（AKA）抗体的IgG类的患者血清中定义的幼年特发性关节炎（JIA）的各种亚组的疾病持续时间超过一年。采用酶联免疫吸附试验（Immunoscan RA，Eurodiagnostica，The Netherlands）和大鼠食管底物间接免疫荧光（IIF）试验（ImmuGloTM，Immco Diagnostics，布法罗，USA）检测和定量140例2-47岁（中位数16.5岁）JIA患者（64例男性和76例女性）的抗CCP和AKA抗体。抗CCP抗体阳性率为5.0%（7/140），其中RF阴性关节炎3/52，RF阳性关节炎2/18，附着点炎相关关节炎1115例，未分类关节炎115例。40/140例患者检测到AKA（28.6%，p = 0.04）包括2/11例全身性关节炎、2/32例少关节炎、18/52例RF阴性多关节炎患者（34.6%，p = 0.01），14/18例RF阳性多发性关节炎（77.8%，p = 0.000002），2/15例附着点炎相关关节炎和2/3例银屑病关节炎。虽然在大多数（97/140; 69.3%）研究病例中同时出现AKA和抗CCP阴性，但仅在少数（4/140; 2.9%）研究样本中发现同时抗体阳性。我们的结论是，虽然AKA测量使用IIF对大鼠食管可以检测到约三分之一的患者明确JIA超过1年的疾病持续时间，只有罕见的发生抗CCP观察。我们的结论是，AKA似乎是部分有用的，以确定JIA的诊断，但没有用于后续的严重性或活动性在JIA患者。与RA相比，抗CCP在JIA队列中没有任何额外的价值，其中报告了其诊断和预后重要性。
We analysed the presence of anti-cyclic citrullinated peptide (anti-CCP) and anti-keratin (AKA) antibodies of the IgG class in sera of patients with defined juvenile idiopathic arthritis (JIA) of various subgroups with more than one year duration of the disease. Enzyme-linked immunosorbent assay (Immunoscan RA, Eurodiagnostica, The Netherlands) and an indirect immunofluorescence (IIF) test on rat oesophagus substrate (ImmuGloTM, Immco Diagnostics, Buffalo, USA) were used for the detection and quantification of anti-CCP and AKA antibodies in 140 patients with JIA (64 male and 76 female) aged 2-47 years (median 16.5 years). Overall, anti-CCP were found in 7/140 (5.0%) patients including 3/52 RF negative polyarthritis, 2/18 RF positive polyarthritis, 1115 enthesitis related arthritis and 115 unclassifiable arthritis. AKA were detected in 40/140 patients (28.6%, p = 0.04) including 2/11 systemic arthritis, 2/32 oligoarthritis, 18/52 patients with RF negative polyarthritis (34.6%, p = 0.01), 14/18 RF positive polyarthritis (77.8%, p = 0.000002), 2/15 enthesitis related arthritis and 2/3 psoriatic arthritis. While simultaneous negativity for AKA and anti-CCP occurred in most (97/140; 69.3%) studied cases, simultaneous antibody positivity was found only in few (4/140; 2.9%) studied samples. We conclude that while AKA measured using IIF on rat esophagus can be detected approximately in one third of patients with definite JIA with more than 1 year duration of the disease, only rare occurrence of anti-CCP was observed. We conclude that AKA seem to be partly useful to confirm JIA diagnosis, however, useless to follow-up severity or activity in JIA patients. Anti-CCP do not have any additional value in JIA cohort in comparison to RA where their diagnostic and prognostic importance was reported.