Altered expression pattern of testican-1 mRNA after brain injury
Altered expression pattern of testican-1 mRNA after brain injury
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DOI：
10.2220/biomedres.32.373
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发表时间：
2011-12-01
影响因子：
1.2
通讯作者：
Tase, Choichiro
中科院分区：
文献类型：
--
作者：
Iseki, Ken;Hagino, Seita;Tase, Choichiro
睾丸素是一种软骨素/硫酸乙酰肝素蛋白聚糖，主要在成年和胚胎小鼠脑的神经元中表达，表明其在神经元的正常和/或增殖和分化过程中的作用。然而，睾丸素在受伤大脑中的作用仍不清楚。在本研究中，我们研究了testican-1 mRNA表达模式冷冻脑损伤后，原位杂交组织化学显示，testican-1 mRNA诱导周围的坏死组织的区域。睾丸素-1 mRNA的时程研究显示，在损伤后7天的信号强度最高水平。为了确定哪些细胞类型表达testican-1 mRNA，我们进行了原位杂交组织化学结合免疫组化的几种细胞标志物。Testican-1 mRNA信号在近端反应性星形胶质细胞中检测到，而testican-1 mRNA阳性细胞的分布模式不同于成熟少突胶质细胞和活化的小胶质细胞。此外，testican-1 mRNA的信号与FGF-2 mRNA的信号重叠，表明这些分子在反应性星形胶质细胞中共表达。这些结果表明，睾丸素-1在损伤后的反应性星形胶质细胞中再生轴突中起允许作用的可能性。
Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain, hi situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.