Parasitism of Pieris rapae (Lepidoptera: Pieridae) by a pupal endoparasitold, Pteromalus puparum (Hymenoptera: Pteromalidae):: effects of parasitization and venom on host hemocytes
Parasitism of Pieris rapae (Lepidoptera: Pieridae) by a pupal endoparasitold, Pteromalus puparum (Hymenoptera: Pteromalidae):: effects of parasitization and venom on host hemocytes
复制标题
DOI：
10.1016/j.jinsphys.2004.01.007
复制
发表时间：
2004-04-01
影响因子：
2.2
通讯作者：
Hu, C
中科院分区：
文献类型：
--
作者：
Cai, J;Ye, GY;Hu, C
与卵幼虫和幼虫内寄生黄蜂的情况相反，人们对蛹内寄生蜂及其分泌物对其昆虫宿主血细胞的影响知之甚少。本研究重点关注蛹内寄生蜂 Pteromalus puparum。及其宿主。小白蝴蝶，菜青虫 (Pieris rapae)。蛹虫草的寄生导致宿主血细胞总数在寄生后第五天显着增加。寄生后第1天至第4天，浆细胞比例明显下降，颗粒细胞比例增加。此外，从寄生后12小时到第三天，被寄生蛹的血细胞死亡率明显更高。当菜粉蝶蛹寄生于经伽马射线照射的成年雌性蛹（假寄生）时，很明显，经过处理的黄蜂可以诱导类似的血细胞变化。然而，这种现象并没有发生在被刺穿的宿主蛹中（模仿寄生）。用蛹蛹毒液处理后。扩散的浆细胞和封装的Sephadex G-25珠的百分比均显着降低；体外。电镜分析和鬼笔环肽-FITC对血细胞F-肌动蛋白的可视化表明，经毒液处理的血细胞具有圆形结构，既不扩散也不延伸伪足，而毒液处理后血细胞骨架没有明显改变。结果表明，蛹蛹毒液可以主动抑制宿主的血细胞免疫反应，但不是通过破坏宿主血细胞的细胞骨架。 (C) 2004 Elsevier Ltd. 保留所有权利。
In contrast to the situation with egg-larval and larval endoparasitic wasps, little is known about the effects Of Pupal endoparasitoids and their secretions on the hemocytes of their insect hosts. This Study focuses on the pupal endoparasitoid, Pteromalus puparum. and its host. the small white butterfly, Pieris rapae. Parasitism by P. puparum, resulted in a significant increase in the total number of host hemocytes up to day five after parasitization. From day one to day four after parasitization, the percentage of plasmatocytes significantly decreased, and the proportion of granular cells increased. Moreover, from 12 h to day three after parasitization, hemocyte mortality in parasitized pupae was noticeably higher. When P. rapae pupae were parasitized by adult females of P. puparum irradiated by gamma-ray (pseudoparasitization), it was clear that the treated wasps could induce similar hemocyte changes. However, such phenomena did not occur in punctured host pupae (mimic-parasitization). After treatment with P. puparum venom. both the percentages of spreading plasmatocytes and encapsulated Sephadex G-25 beads were lessened significantly; in vitro. Electron microscopy analysis and visualization of hemocyte F-actin with phalloidin-FITC showed that hemocytes treated with venom had a rounded configuration and neither spread nor extended pseudopods, while there was no marked alteration of hemocvte cytoskeletons after venom treatment. The results suggested that venom of P. puparum could actively suppress the hemocyte immune response of its host, but not by destroying the host hemocyte cytoskeleton. (C) 2004 Elsevier Ltd. All rights reserved.